U-Erlenmeyer Omangele Shake Flask onekepisi elikhipha umoya
Isici
1. Ngokusho kokukhiqizwa okujwayelekile kwe-c-GMP, akukho ukuxhumana komuntu siqu, ukungaguquguquki okukhulu.
2. Isivalo sebhodlela sisebenzisa izinto eziqinile ze-HDPE futhi siklanywe nge-PTFE hydrophobic kanye nolwelwesi oluphefumulayo. Ngemva kokuthintana noketshezi, ngeke kuthinte umphumela wokuvala nokukhipha umoya kolwelwesi oluphefumulayo.
3. Isikali sicacile futhi sinembile, esilungele ukubheka umthamo ophakathi nendawo
4.Amandla amane we-125ml, 250ml, 500ml kanye ne-1000ml
5. Ukupakishwa komuntu ngamunye kwe-Aseptic
Umehluko phakathi kwe-baffle shake flask ne-conical erlenmeyer flask evamile
Esikhathini sokuthuthuka okusheshayo kobuchwepheshe obuhlukahlukene, izinto ezisetshenziswayo zesiko lamaseli nazo zibuyekezwa njalo futhi ziphindaphindwe, futhi i-baffle shaker iyisiko lamaseli anoveli elisebenzisekayo.Uyini umehluko phakathi kwamaflaski amabili ajwayelekile?
Okokuqala, mayelana nokuma, bobabili bamukela umklamo ongunxantathu, futhi izigqoko zamabhodlela nazo zihlukaniswe zibe izinhlobo ezimbili: ama-caps avaliwe nama-caps aphefumulayo, futhi ukucaciswa kucishe kufane.Umehluko omkhulu phakathi kwalokhu okubili phansi kwebhodlela.Iphansi le-shaker elijwayelekile liyisicaba, kanti ngaphansi kwe-baffle shaker kunemifantu.Izingxenye eziphakanyisiwe zale misele zenza ukudideka ngaphakathi kwebhodlela, ngakho-ke igama.
Umklamo okhethekile we-baffle flask unemisebenzi emibili.Enye iwukunciphisa ukwenzeka kwe-cell clumping.Ukuqhaqhazela nge-shaker kunganciphisa ngempumelelo i-viscosity ebangelwa i-DNA yamahhala kanye nemfucumfucu yamaseli, futhi kunciphise ukuvela kokukhula kwamaseli.Ngaphezu kwalokho, i-baffle engezansi ingaphinde ivimbele i-vortex phenomenon eyenziwa yi-medium ngesikhathi sokuthuthumela, okwenza i-medium ibe yinto efanayo, ebuye ibe nomthelela othile ekunciphiseni i-cell clumping.Okwesibili ukukhulisa inani le-oxygen encibilikile.I-baffle engaphansi kwebhodlela ingakhuphula inani le-oxygen encibilikile phakathi nendawo, ikhuthaze ngokuphumelelayo ukuxhumana okugcwele phakathi kwamaseli nomoya, futhi isize amangqamuzana akhule kangcono.
Ngokuvamile, umehluko omkhulu phakathi kwe-baffle shake flasks kanyeama-shake flasks ajwayelekileumehluko ongaphansi kwebhodlela.Uhlobo olusha lwebhodlela lukhulisa inani le-oxygen encibilikile futhi lufaneleka kakhulu imigqa yamaseli edinga inani elikhulu le-oxygen.
Izici ezimbili ze-baffled shaker erlenmeyer flask
1. Yehlisa ukunqwabelana kwamaseli
Enqubweni ye-suspension cell culture, ukukhula kwe-cell clumping kuvame ukuhlangana.Izizathu zihlukahlukene, njengokuntuleka kokumiswa kabusha ngemuva kwe-centrifugation, noma inkinga ye-serum phakathi nendawo, noma ukugcwala kwe-calcium ne-magnesium ion.izinguquko ekunamatheleni phakathi kwamaseli.I-baffle flask inyakaziswa nge-shaker, enganciphisa ngempumelelo i-viscosity ebangelwa i-DNA yamahhala kanye nemfucumfucu yamangqamuzana, futhi inciphise ukwenzeka kokukhula kwamaseli.Ngaphezu kwalokho, i-baffle engezansi ingaphinde ivimbele i-vortex phenomenon eyenziwa yi-medium ngesikhathi sokuzamazama, okwenza i-medium ibe iyunifomu eyengeziwe, ebuye inciphise ithuba lokuhlangana kweseli ngezinga elithile.
2. Khulisa umoya-mpilo oncibilikisiwe
Ikepisi eliphefumulayo liyisiteshi esibalulekile sokushintshaniswa kwegesi yebhodlela le-baffle shaker.Ngomsebenzi ophefumulayo we-membrane yokuphefumula, ngakolunye uhlangothi, ingakhuthaza ukushintshaniswa kwegesi ebhodleleni, futhi ngakolunye uhlangothi, ingavimbela ngokuphumelelayo ukungcoliswa kwama-microorganisms.I-baffle engaphansi kwebhodlela ingakhuphula inani le-oxygen encibilikile endaweni yesiko, ikhuthaze ngokuphumelelayo ukuxhumana phakathi kwamaseli nomoya, ngaleyo ndlela ithuthukise ukusebenza kahle kokushintshaniswa kwegesi futhi ivumele amaseli ukuthi akhule kangcono.
Umklamo okhethekile we-baffle flask ikakhulukazi ngenxa yokugoqa phansi kwebhodlela, okunciphisa ukuhlangana kwamaseli, ukwandisa umoya-mpilo oncibilikisiwe, futhi unikeze izimo ezinhle zokukhula kwamaseli.
● Ipharamitha Yomkhiqizo
| Isigaba | Inombolo ye-athikili | Ivolumu | Cap | Okubalulekile | Ukucaciswa kwephakheji | Ubukhulu bebhokisi |
| I-Baffled Erlenmeyer flask, PETG | LR036125 | 125ml | uphawu Cap | I-PETG,Ukuvala inzalo ngemisebe | 1pcs/pack24pack/case | 31 X 21 X 22 |
| LR036250 | 250ml | 1pcs/pack12pack/case | 31 X 21 X 22 | |||
| LR036500 | 500ml | 1pcs/pack12pack/case | 43 X 32 X 22 | |||
| LR036001 | 1000ml | 1pcs/pack12pack/case | 55 X 33.7 X 24.5 | |||
| I-Baffled Erlenmeyer flask, PETG | LR037125 | 125ml | I-Vent Cap | I-PETG,Ukuvala inzalo ngemisebe | 1pcs/pack24pack/case | 31 X 21 X 22 |
| LR037250 | 250ml | 1pcs/pack12pack/case | 31 X 21 X 22 | |||
| LR037500 | 500ml | 1pcs/pack12pack/case | 43 X 32 X 22 | |||
| LR037001 | 1000ml | 1pcs/pack12pack/case | 55 X 33.7 X 24.5 | |||
| I-Baffled Erlenmeyer flask, i-PC | LR034125 | 125ml | uphawu Cap | I-PC, inzalo yokushiswa kwemisebe | 1pcs/pack24pack/case | 31 X 21 X 22 |
| LR034250 | 250ml | 1pcs/pack12pack/case | 31 X 21 X 22 | |||
| LR034500 | 500ml | 1pcs/pack12pack/case | 43 X 32 X 22 | |||
| LR034001 | 1000ml | 1pcs/pack12pack/case | 55 X 33.7 X 24.5 | |||
| I-Baffled Erlenmeyer flask, i-PC | LR035125 | 125ml | I-Vent Cap | I-PC, inzalo yokushiswa kwemisebe | 1pcs/pack24pack/case | 31 X 21 X 22 |
| LR035250 | 250ml | 1pcs/pack12pack/case | 31 X 21 X 22 | |||
| LR035500 | 500ml | 1pcs/pack12pack/case | 43 X 32 X 22 | |||
| LR035001 | 1000ml | 1pcs/pack12pack/case | 55 X 33.7 X 24.5 |
