• lab-217043_1280

U-Erlenmeyer Omangele Shake Flask onekepisi elikhipha umoya

Ngokwesiko lamaseli amisiwe, i-erlenmeyer shake flasks ayizinto ezisetshenziswayo ezivame ukusetshenziswa.Ngokushintsha njalo kwezidingo zemakethe, kwavela ibhodlela eliklanywe ngokukhethekile - i-cell shaker edidekile.Ngakho-ke, yiziphi izici zaleli bhodlela, futhi yiziphi izincazelo ezivamile?

Kuhlukile kwi amaflaski e-erlenmeyer shaker ane-flat-bottomed ajwayelekile, ama- baffled cell shaker flasks ayimikhumbi ye-cell culture enezincezu ngezansi kwe-flask, ezisetshenziselwa ikakhulukazi isiko lamaseli amisiwe anezidingo eziphezulu ze-oxygen.Leli bhodlela lisebenzisa ukwakheka kwebhodlela elingaphansi futhi lilinyakazisa nge-shaker ngesikhathi sokusetshenziswa, elinganciphisa ngempumelelo i-viscosity ebangelwa i-DNA yamahhala kanye nemfucumfucu yamaseli, ngaleyo ndlela kunciphise ukukhula kwe-cell clumping.Ngaphezu kwalokho, i-baffle engaphansi kwebhodlela ingandisa inani le-oxygen encibilikile endaweni yesiko, futhi ngokusebenzisa isembozo esiphefumulayo, ingakhuthaza ngokuphumelelayo ukuxhumana phakathi kwamaseli nomoya, futhi inikeze izimo ezinhle zegesi zeseli. ukukhula.

 

Ukuze uthole imininingwane eyengeziwe noma amasampula wamahhala sicela ukhululeke ukuxhumana nathi.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

I-Plastiki Erlenmeyer Shake Flask

Isici
1. Ngokusho kokukhiqizwa okujwayelekile kwe-c-GMP, akukho ukuxhumana komuntu siqu, ukungaguquguquki okukhulu.
2. Isivalo sebhodlela sisebenzisa izinto eziqinile ze-HDPE futhi siklanywe nge-PTFE hydrophobic kanye nolwelwesi oluphefumulayo. Ngemva kokuthintana noketshezi, ngeke kuthinte umphumela wokuvala nokukhipha umoya kolwelwesi oluphefumulayo.
3. Isikali sicacile futhi sinembile, esilungele ukubheka umthamo ophakathi nendawo

4.Amandla amane we-125ml, 250ml, 500ml kanye ne-1000ml

5. Ukupakishwa komuntu ngamunye kwe-Aseptic

Umehluko phakathi kwe-baffle shake flask ne-conical erlenmeyer flask evamile
Esikhathini sokuthuthuka okusheshayo kobuchwepheshe obuhlukahlukene, izinto ezisetshenziswayo zesiko lamaseli nazo zibuyekezwa njalo futhi ziphindaphindwe, futhi i-baffle shaker iyisiko lamaseli anoveli elisebenzisekayo.Uyini umehluko phakathi kwamaflaski amabili ajwayelekile?
Okokuqala, mayelana nokuma, bobabili bamukela umklamo ongunxantathu, futhi izigqoko zamabhodlela nazo zihlukaniswe zibe izinhlobo ezimbili: ama-caps avaliwe nama-caps aphefumulayo, futhi ukucaciswa kucishe kufane.Umehluko omkhulu phakathi kwalokhu okubili phansi kwebhodlela.Iphansi le-shaker elijwayelekile liyisicaba, kanti ngaphansi kwe-baffle shaker kunemifantu.Izingxenye eziphakanyisiwe zale misele zenza ukudideka ngaphakathi kwebhodlela, ngakho-ke igama.
Umklamo okhethekile we-baffle flask unemisebenzi emibili.Enye iwukunciphisa ukwenzeka kwe-cell clumping.Ukuqhaqhazela nge-shaker kunganciphisa ngempumelelo i-viscosity ebangelwa i-DNA yamahhala kanye nemfucumfucu yamaseli, futhi kunciphise ukuvela kokukhula kwamaseli.Ngaphezu kwalokho, i-baffle engezansi ingaphinde ivimbele i-vortex phenomenon eyenziwa yi-medium ngesikhathi sokuthuthumela, okwenza i-medium ibe yinto efanayo, ebuye ibe nomthelela othile ekunciphiseni i-cell clumping.Okwesibili ukukhulisa inani le-oxygen encibilikile.I-baffle engaphansi kwebhodlela ingakhuphula inani le-oxygen encibilikile phakathi nendawo, ikhuthaze ngokuphumelelayo ukuxhumana okugcwele phakathi kwamaseli nomoya, futhi isize amangqamuzana akhule kangcono.

Ngokuvamile, umehluko omkhulu phakathi kwe-baffle shake flasks kanyeama-shake flasks ajwayelekileumehluko ongaphansi kwebhodlela.Uhlobo olusha lwebhodlela lukhulisa inani le-oxygen encibilikile futhi lufaneleka kakhulu imigqa yamaseli edinga inani elikhulu le-oxygen.

Izici ezimbili ze-baffled shaker erlenmeyer flask

1. Yehlisa ukunqwabelana kwamaseli
Enqubweni ye-suspension cell culture, ukukhula kwe-cell clumping kuvame ukuhlangana.Izizathu zihlukahlukene, njengokuntuleka kokumiswa kabusha ngemuva kwe-centrifugation, noma inkinga ye-serum phakathi nendawo, noma ukugcwala kwe-calcium ne-magnesium ion.izinguquko ekunamatheleni phakathi kwamaseli.I-baffle flask inyakaziswa nge-shaker, enganciphisa ngempumelelo i-viscosity ebangelwa i-DNA yamahhala kanye nemfucumfucu yamangqamuzana, futhi inciphise ukwenzeka kokukhula kwamaseli.Ngaphezu kwalokho, i-baffle engezansi ingaphinde ivimbele i-vortex phenomenon eyenziwa yi-medium ngesikhathi sokuzamazama, okwenza i-medium ibe iyunifomu eyengeziwe, ebuye inciphise ithuba lokuhlangana kweseli ngezinga elithile.
2. Khulisa umoya-mpilo oncibilikisiwe
Ikepisi eliphefumulayo liyisiteshi esibalulekile sokushintshaniswa kwegesi yebhodlela le-baffle shaker.Ngomsebenzi ophefumulayo we-membrane yokuphefumula, ngakolunye uhlangothi, ingakhuthaza ukushintshaniswa kwegesi ebhodleleni, futhi ngakolunye uhlangothi, ingavimbela ngokuphumelelayo ukungcoliswa kwama-microorganisms.I-baffle engaphansi kwebhodlela ingakhuphula inani le-oxygen encibilikile endaweni yesiko, ikhuthaze ngokuphumelelayo ukuxhumana phakathi kwamaseli nomoya, ngaleyo ndlela ithuthukise ukusebenza kahle kokushintshaniswa kwegesi futhi ivumele amaseli ukuthi akhule kangcono.

Umklamo okhethekile we-baffle flask ikakhulukazi ngenxa yokugoqa phansi kwebhodlela, okunciphisa ukuhlangana kwamaseli, ukwandisa umoya-mpilo oncibilikisiwe, futhi unikeze izimo ezinhle zokukhula kwamaseli.

● Ipharamitha Yomkhiqizo

 

Isigaba Inombolo ye-athikili Ivolumu Cap Okubalulekile Ukucaciswa kwephakheji Ubukhulu bebhokisi
I-Baffled Erlenmeyer flask, PETG LR036125 125ml uphawu Cap I-PETG,Ukuvala inzalo ngemisebe 1pcs/pack24pack/case 31 X 21 X 22
LR036250 250ml 1pcs/pack12pack/case 31 X 21 X 22
LR036500 500ml 1pcs/pack12pack/case 43 X 32 X 22
LR036001 1000ml 1pcs/pack12pack/case 55 X 33.7 X 24.5
I-Baffled Erlenmeyer flask, PETG LR037125 125ml I-Vent Cap I-PETG,Ukuvala inzalo ngemisebe 1pcs/pack24pack/case 31 X 21 X 22
LR037250 250ml 1pcs/pack12pack/case 31 X 21 X 22
LR037500 500ml 1pcs/pack12pack/case 43 X 32 X 22
LR037001 1000ml 1pcs/pack12pack/case 55 X 33.7 X 24.5
I-Baffled Erlenmeyer flask, i-PC LR034125 125ml uphawu Cap

I-PC, inzalo yokushiswa kwemisebe

1pcs/pack24pack/case 31 X 21 X 22
LR034250 250ml 1pcs/pack12pack/case 31 X 21 X 22
LR034500 500ml 1pcs/pack12pack/case 43 X 32 X 22
LR034001 1000ml 1pcs/pack12pack/case 55 X 33.7 X 24.5
I-Baffled Erlenmeyer flask, i-PC LR035125 125ml I-Vent Cap I-PC, inzalo yokushiswa kwemisebe 1pcs/pack24pack/case 31 X 21 X 22
LR035250 250ml 1pcs/pack12pack/case 31 X 21 X 22
LR035500 500ml 1pcs/pack12pack/case 43 X 32 X 22
LR035001 1000ml 1pcs/pack12pack/case 55 X 33.7 X 24.5

 

 


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