Ipulasitiki i-Erlenmeyer Shake Flask enekepisi yokukhipha umoya Isithombe Esifakiwe

I-Plastiki i-Erlenmeyer Shake Flask enekepisi yokukhipha umoya

Ii-erlenmeyer flaskifanele isiko lemigqa yamaseli enezidingo eziphezulu zomoya-mpilo, futhi ingasetshenziselwa isiko lamabhaktheriya, isikhunta namaseli ezilwane nezitshalo ekumisweni.Uma kuqhathaniswa neflask yesiko, isitsha neflask ejikelezayo, kudinga umsebenzi omncane.Kuyithuluzi elinomnotho lesikompilo lamaseli, futhi iyinketho efanelekile yokulungiselela okuphakathi, ukuxuba nokugcinwa,I-PETG / PC esobala ephezulu iqinile futhi akulula ukuyiphula, enganciphisa ingozi efihliwe yokuphepha komuntu siqu.Kuyisinqumo esihle sohlelo lokusebenza lokunyakazisa isiko.Ukuze uthole amasampula mahhala sicela ukhululeke ukuxhumana nathi.

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Imininingwane Yomkhiqizo

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I-Erlenmeyer Flask enekepisi yokukhipha umoya

Isici se-Erlenmeyer Shake Flask

I-erlenmeyer flask, eyaziwa nangokuthi i-triangular shake flask, ifaneleka kakhulu ukutshalwa kwemigqa yamaseli ezinambuzane ezinezidingo eziphezulu ze-oxygen.Uma kuqhathaniswa nezinto ezisetshenziswayo ezifana namafekthri amaseli kanye nama-cell spinner flasks, indawo yesiko lamaseli incane futhi iyithuluzi lezomnotho lamaseli..
Umzimba weflask wenziwe nge-polycarbonate (PC) noma i-PETG impahla.Idizayini eyingqayizivele yesimo esingunxantathu yenza kube lula ukuthi ama-pipette noma ama-cell scrapers afinyelele ekhoneni leflask, okwenza imisebenzi yesiko lamaseli ibe lula kakhulu.Isigqoko sebhodlela senziwe ngezinto eziqinile ze-HDPE, ezihlukaniswe zibe isivalo sokuvala kanye nesigqoko esiphefumulayo.I-cap yokuvala isetshenziselwa isiko elivaliwe legesi noketshezi.I-vent cap ifakwe ulwelwesi lwesihlungi se-hydrophobic phezulu kwekepisi yebhodlela.Ivimbela ukungena nokuphuma kwama-microorganisms, ivimbela ukungcola, futhi iqinisekise ukushintshaniswa kwegesi, ukuze amaseli noma amagciwane akhule kahle.

I-flask yesiko elingunxantathu lakhiwa umzimba webhodlela kanye nesigqoko sebhodlela .. Umklamo oyingqayizivele webhodlela lebhodlela lenza kube lula ukuthi ama-pipette noma ama-cell scrapers afinyelele ekhoneni lebhodlela, okwandisa ukunethezeka kokusebenza kwesiko lamaseli.kanye nokuzinza.Osayizi abajwayelekile bamaflask okunyakazisa angunxantathu ngu-125ml, 250ml, 500ml kanye no-1000ml.Ukuze ubuke umthamo we-medium futhi ubambe isimo sokukhula samaseli, isikali sizophrintwa emzimbeni webhodlela.Inqubo yeseli idinga ukwenziwa endaweni eyinyumba.Ngakho-ke, i-Erlenmeyer flask izophathwa ngokukhethekile inzalo ngaphambi kokuba isetshenziswe ukuze kuzuzwe umphumela wokungabi bikho kwe-DNase, i-RNase, kanye nezithako ezitholakala ezilwaneni, ezihlinzeka ngezimo ezinhle zokukhula kwamaseli.indawo ezungezile.

U-Erlenmeyer Shake onekepisi elikhipha umoya

Amaseli Akhula Kancane Kancane ku-Erlenmeyer Flask nesixazululo

Yini edala ukukhula kancane kwamaseli kumaflask e-cell shaker
Amaseli azwela kakhulu endaweni yokukhula.Lapho sihlakulela amangqamuzana, ngezinye izikhathi sibhekana nokukhula kwamaseli okuhamba kancane.Siyini isizathu?Kunezizathu eziningi zokukhula kancane kwamaseli ku-cell shake flask, ikakhulukazi ngenxa yalezi zizathu ezilandelayo:
1. Amaseli adinga ukulungiswa kabusha ngenxa yokushintsha kwesiko elihlukile noma i-serum.
2. Ama-reagents agcinwa ngendlela engafanele, futhi ezinye izingxenye ezidingekayo zokukhula kwamaseli njenge-glutamine noma izici zokukhula endaweni yesiko ziphelile noma azikho noma zicekelwe phansi.
3. Kukhona inani elincane lokungcola kwebhaktheriya noma ukukhunta esikweni ku-cell shaker.
4. Iqoqo lokuqala lamaseli afakwe i-inoculated liphansi kakhulu.
5. Amaseli asekhulile.
6. Ukungcola kwe-Mycoplasma
Isixazululo esiphakanyisiwe:
1. Qhathanisa ukwakheka kwendlela entsha kanye nendlela yokuqala, bese uqhathanisa i-serum entsha neserum endala ukuze usekele ukuhlolwa kokukhula kwamaseli.Vumela amaseli ukuthi ajwayelane kancane kancane nendawo entsha.
2. Shintshela endaweni yesiko elisanda kulungiswa, noma wengeze i-glutamine nezici zokukhula.
3. Fukamisa nge-antibiotic-free medium bese ubuyisela isiko uma ukungcola kutholakala.I-culture medium kufanele igcinwe ku-2-8 ° C ebumnyameni.Isilinganiso esiphelele esiqukethe i-serum sigcinwa ku-2-8°C futhi sisetshenziswe phakathi kwamaviki ama-2.
4. Khulisa ukugxila kokuqala kwamaseli afakwe i-inoculated.
5. Faka esikhundleni ngamaseli amasha anembewu.
6. Hlukanisa isiko futhi uthole i-mycoplasma.Hlanza i-stand kanye ne-incubator.Uma ukungcoliswa kwe-mycoplasma kutholakala, buyisela isiko elisha.

● Ipharamitha Yomkhiqizo

Isigaba	Inombolo ye-athikili	Ivolumu	Cap	Okubalulekile	Ukucaciswa kwephakheji	Ubukhulu bebhokisi
Erlenmeyer flask, PETG	LR030125	125ml	uphawu Cap	I-PETG,Ukuvala inzalo ngemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR030250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR030500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR030001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
Erlenmeyer flask, PETG	LR031125	125ml	I-Vent Cap	I-PETG,Ukuvala inzalo ngemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR031250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR031500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR031001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, i-PC	LR032125	125ml	uphawu Cap	I-PC, inzalo yokushiswa kwemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR032250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR032500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR032001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, i-PC	LR033125	125ml	I-Vent Cap	I-PC, inzalo yokushiswa kwemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR033250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR033500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR033001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5