Isihloko esandulele sethule izizathu ezimbalwa zokuthi kungani amaseli anganamatheli odongeni lapho esebenzisaama-cell culture flasksnezinye iziqukathi.Ngakho-ke yini okufanele yenziwe uma umphumela wesiko lokubambelela kumaseli muhle?Ake ngikunikeze isingeniso esifushane.

Uma ufuna umphumela wesiko lokunamathela kumaseli ube muhle, kufanele wenze lokhu okulandelayo:

1. Gaya amangqamuzana ngokulingene;

2. Kunconywa ukuthi kusetshenziswe iziqukathi zepulasitiki ezilahlwa phezulu ezilahlwayo ukuze kulinywe;

3. Sebenzisa i-matrix ye-extracellular efanelekile noma ama-reagents okunamathiselwe;

4. Vuselela amaseli amasha, sebenzisa i-serum engu-20% ngesonto lokuqala ukuze usize amangqamuzana alulame;

5. Ukujova kwe-subculture kumele kusatshalaliswe ngokulinganayo ukugwema ukukhula kweqoqo lamaseli;

6. Zama ukunganyakazi amaseli kungakapheli amahora angama-24 we-subculture, ukuze ungaphazamisi ukunamathela.