I-Liquid strain iwuhlobo olukhuliswe nge-liquid culture medium.Inezinzuzo zomjikelezo wokukhiqiza we-strain, iminyaka yebhaktheriya engaguquki, i-inoculation elula, efanelekile ukukhiqizwa kwefekthri, njalonjalo.Yamukelwe iningi labalimi.I-cell shaker (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） iwuhlobo lwezinto ezisebenzisekayo ezidingekayo kusiko lwezinhlobo eziwuketshezi.Amaphuzu alandelayo adinga ukunakwa kusiko:

1. Ukulungiswa kwesiko eliphakathi ngokwefomula yezingxenye ezihlukahlukene, esitsheni, chaza ngamanzi, ahlukaniswe amabhodlela angunxantathu, ngokuvamile 250 ~ 300mL cell shake ibhodlela 50mL isiko medium,500mL ibhodlela elingunxantathu  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100mL isiko medium, ipulaki ukotini ipulaki, nephephandaba esongwe;Ingenziwa futhi ngezingqimba eziyi-8 zegauze engu-8cm×8cm yokuvala isivalo sebhodlela, ngaphandle ngokuvalwa kwephepha elinsundu.
2. Indlela yokuvala inzalo ye-Autoclave ngokuvamile isetshenziselwa ukuvala inzalo, okudinga u-121℃ kanye nemizuzu engama-30.Yikhiphe bese uyipholisa ifike ku-30℃.Yibeke ekamelweni eliyinyumba noma ibhokisi lokugomela ukuze lisetshenziswe.
3. Ngokwezidingo zokusebenza kwe-aseptic, ibhodlela ngalinye lingaxhunywa nohlobo lwe-slant 2 ~ 3cm2, futhi uhlobo ngalunye lwe-slant lungaxhunywa namabhodlela angu-4 ~ 5.Kungcono ukuletha i-medium ne-strains eyengeziwe, ukuze ikwazi ukuntanta ebusweni be-medium.Ngemuva kokujova, vala umlomo webhodlela nge-gauze exhunywe ekuqaleni, futhi uyibophe ngokuqinile ngentambo.
4. Ibhodlela le-strain elifakiwe lingafakwa embhedeni onyakaziswayo ngenxa yesiko, noma lingafakwa ekushiseni okungaguquki okungama-24 ~ 26℃ ama-48h, bese isiko elixutshiwe lingenziwa ngemuva kokuthi i-mycelium ye-aerogenic inwetshwe endaweni yesiko. .Imvamisa ye-oscillating ye-shaker ephindaphindayo ingu-80 ~ 120r/min, futhi leyo ye-rotary shaker ingu-150 ~ 220r/min.I-3 ~ 4d ingakhuliswa ku-shaker.Ekupheleni kwesiko, ngenxa yezinhlobo ezahlukene, i-culture medium yavela ngemibala ehlukene, njenge-oyster mushroom, i-enok mushroom culture medium yayiphuzi ngokukhanyayo;Amakhowe, i-ericius erinaceus culture medium yayibomvu ngokubomvu, nephunga lamakhowe;Isiko lesikhunta sinsundu, siwugqinsi futhi simnandi.I-culture medium ingasetshenziselwa ukukhiqiza noma isiko lokunwetshwa okwengeziwe kuphela lapho isisindo esomile se-mycelium sifinyelela ku-10g/L futhi ububanzi be-mycelium pellets ingu-1 ~ 2mm.
 
5. Ukukhiqizwa kohlobo lwesibili lwe-liquid strain yesibili isiko ukukhiqizwa okuphakathi kwezinga elifanayo lohlobo lwe-liquid, isitsha sesiko kufanele sibe sikhulu, umthamo awudluli ku-3500mL.Ngemuva kokuvala inzalo nokupholisa, izinhlobonhlobo zamanzi ezivutshiwe zifakwa kuzo5L cell shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）ngesilinganiso sika-5% -10% futhi ikhule ku-shaker ngesivinini esincane.Ngemuva kwesikhathi esithile sesiko le-oscillating, i-liquid strains enokusabalalisa okufanayo kwama-microspheres kanye noketshezi olucacile lokuvutshelwa lungatholakala.
Kafushane, lapho usebenzisa cell shaker ukuze isiko liquid amagciwane, ukusebenza ngokuvumelana nezindlela ezingenhla, futhi unake ukulawula izinga lokushisa, unyakazisa imvamisa kanye nezinye izici inqubo ukuqinisekisa umphumela isiko.
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