I-vacuolation yamaseli ibhekisela ekubonakaleni kwama-vacuoles (ama-vesicles) osayizi abahlukene ku-cytoplasm kanye ne-nucleus yamaseli awonakele, futhi amaseli angamaselula noma ama-reticular.Ziningi izizathu zalesi simo.Singanciphisa i-vacuolation yamaseli kui-cell culture flaskkancane ngangokunokwenzeka ngokusebenzisa imisebenzi yansuku zonke.
1. Qinisekisa isimo seseli: nquma isimo seseli ngaphambi kokuhlakulela amaseli, bese uzama ukukhetha amaseli anenombolo yesizukulwane esiphezulu ukuze alinywe, ukuze ugweme ama-vacuoles ngenxa yokuguga kwamaseli ngesikhathi sokutshala.

2. Thola ivelu ye-pH yendawo yesiko: qinisekisa ukufaneleka kwe-pH yendawo yesiko kanye ne-pH edingwa amaseli ukugwema ukuthikameza ukukhula kwamaseli ngenxa ye-pH engalungile.
3. Lawula isikhathi sokugayeka kwe-trypsin: uma ukhiqiza i-trypsin, khetha ukugxila okufanele kwe-trypsin bese ukhetha isikhathi esifanele sokugaya sokugaya, futhi ugweme amabhamuza amaningi kakhulu ngesikhathi sokusebenza.
4. Qaphela isimo seseli nganoma yisiphi isikhathi: Lapho uhlakulela amangqamuzana, bheka isimo seseli kuflask yesiko lamaseli nganoma yisiphi isikhathi ukuze uqinisekise ukuthi amangqamuzana adinga imisoco eyanele futhi agweme ukukhishwa kwamaseli ngenxa yokuntuleka kwezakhi.
5. Zama ukusebenzisa i-fetal bovine serum enekhwalithi enhle nemigudu evamile, ngoba i-serum enjalo inothile ngezakhi futhi inezici ezimbalwa ezikhuthazayo zangaphandle, ezingagwema ngokuphumelelayo izinkinga ezinjalo.
Imisebenzi engenhla inganciphisa ukuvuleka kwamaseli kuflask yesiko lamaseli.Ngaphezu kwalokho, izidingo zobunyumba kufanele zisetshenziswe ngokuqinile ngesikhathi sokusebenza ukuze kuncishiswe amathuba okungcola okuhlukahlukene.Uma amaseli etholakala engcolile, kufanele alahlwe ngesikhathi ukuze kugwenywe ukuthikameza ukuhlolwa okulandelayo.