I-vacuolation yamaseli ibhekisela ekubonakaleni kwama-vacuoles (ama-vesicles) osayizi abahlukene ku-cytoplasm kanye ne-nucleus yamaseli awonakele, futhi amaseli angamaselula noma ama-reticular.Ziningi izizathu zalesi simo.Singanciphisa i-vacuolation yamaseli kui-cell culture flaskkancane ngangokunokwenzeka ngokusebenzisa imisebenzi yansuku zonke.

1. Qinisekisa isimo seseli: nquma isimo seseli ngaphambi kokuhlakulela amaseli, bese uzama ukukhetha amaseli anenombolo yesizukulwane esiphezulu ukuze alinywe, ukuze ugweme ama-vacuoles ngenxa yokuguga kwamaseli ngesikhathi sokutshala.

2. Thola ivelu ye-pH yendawo yesiko: qinisekisa ukufaneleka kwe-pH yendawo yesiko kanye ne-pH edingwa amaseli ukugwema ukuthikameza ukukhula kwamaseli ngenxa ye-pH engalungile.

3. Lawula isikhathi sokugayeka kwe-trypsin: uma ukhiqiza i-trypsin, khetha ukugxila okufanele kwe-trypsin bese ukhetha isikhathi esifanele sokugaya sokugaya, futhi ugweme amabhamuza amaningi kakhulu ngesikhathi sokusebenza.

4. Qaphela isimo seseli nganoma yisiphi isikhathi: Lapho uhlakulela amaseli, bheka isimo seseli kui-cell culture flasknganoma yisiphi isikhathi ukuqinisekisa ukuthi amangqamuzana adinga izakhi ezanele futhi agweme i-cell vacuolization ngenxa yokuntuleka kwezakhi.

5. Zama ukusebenzisa i-fetal bovine serum enekhwalithi enhle nemigudu evamile, ngoba i-serum enjalo inothile ngezakhi futhi inezici ezimbalwa ezikhuthazayo zangaphandle, ezingagwema ngokuphumelelayo izinkinga ezinjalo.

Imisebenzi engenhla inganciphisa ukuvuleka kwamaseli kui-cell culture flask.Ngaphezu kwalokho, izidingo zobunyumba kufanele zisetshenziswe ngokuqinile ngesikhathi sokusebenza ukuze kuncishiswe amathuba okungcola okuhlukahlukene.Uma amaseli etholakala engcolile, kufanele alahlwe ngesikhathi ukuze kugwenywe ukuthikameza ukuhlolwa okulandelayo.

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