• lab-217043_1280

Hlola izicelo ezintathu PETG medium amabhodlela

PETG isiko medium ibhodlelaiyibhodlela lepulasitiki elisetshenziswa kakhulu.Umzimba webhodlela layo usobala kakhulu, wamukela ukwakheka kwesikwele, isisindo esilula, futhi akulula ukuphuka.Kuyisitsha esihle sokugcina.Izicelo zethu ezijwayelekile ikakhulukazi yilezi ezilandelayo ezintathu:

1. I-Serum: I-Serum inikeza amangqamuzana ngemisoco eyisisekelo, izici zokukhula, amaprotheni abophayo, njll., ukugwema ukulimala kwemishini kumaseli, nokuvikela amaseli esikweni.I-Serum yokugcina isikhathi eside kufanele igcinwe endaweni yokushisa ephansi engu-20°C kuya ku-70°C.Uma igcinwe esiqandisini esingu-4°C, ngokuvamile asikho ngaphezu kwenyanga engu-1.

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2.Isiko lamasiko: Indawo yesiko ngokuvamile iqukethe ama-carbohydrates, izinto ezine-nitrogen, usawoti we-inorganic, amavithamini namanzi, njll. Akuyona nje into eyisisekelo yokuhlinzeka ngokudla kwamangqamuzana nokukhuthaza ukwanda kwamangqamuzana, kodwa futhi nendawo yokuhlala yokukhula nokukhiqizwa kwamangqamuzana. .Indawo yokugcina okuphakathi ingu-2°C-8°C, ivikelekile ekukhanyeni.

3. Ama-reagents ahlukahlukene: Ngaphezu kokugcinwa kwe-serum ne-culture medium, amabhodlela aphakathi kwe-PETG angasetshenziswa njengeziqukathi zokugcina ama-reagents ahlukahlukene ezinto eziphilayo, njengama-buffers, ama-dissociation reagents, ama-antibiotics, izixazululo ze-cell cryopreservation, izixazululo zombala, izithasiselo zokukhula, njll. Amanye alawa ma-reagents adinga ukugcinwa ku--20 ° C, kanti amanye agcinwe ekamelweni lokushisa.Kungakhathaliseki ukuthi iyiphi indawo ezungezile, ibhodlela eliphakathi lingahlangabezana nezidingo zabo zokulondoloza.

I PETG medium ibhodlela ngokuyinhloko esetshenziswa ukubamba ngenhla izixazululo ezintathu.Ukuze kube lula ukubhekwa okubonakalayo komthamo wesixazululo, kunesilinganiso emzimbeni webhodlela.Izixazululo ezingenhla zisetshenziswa ngokuyisisekelo kusiko lamaseli, futhi kufanele kuqashelwe ekusebenzeni kwe-aseptic lapho wengeza.


Isikhathi sokuthumela: Aug-02-2022