PETG isiko medium ibhodlelaiyibhodlela lepulasitiki elisetshenziswa kakhulu.Umzimba webhodlela layo usobala kakhulu, wamukela ukwakheka kwesikwele, isisindo esilula, futhi akulula ukuphuka.Kuyisitsha esihle sokugcina.Izicelo zethu ezijwayelekile ikakhulukazi yilezi ezilandelayo ezintathu:

1. I-Serum: I-Serum inikeza amangqamuzana ngemisoco eyisisekelo, izici zokukhula, amaprotheni abophayo, njll., ukugwema ukulimala kwemishini kumaseli, nokuvikela amaseli esikweni.I-Serum yokugcina isikhathi eside kufanele igcinwe endaweni yokushisa ephansi engu-20°C kuya ku-70°C.Uma igcinwe esiqandisini esingu-4°C, ngokuvamile asikho ngaphezu kwenyanga engu-1.

2.Isiko lamasiko: Indawo yesiko ngokuvamile iqukethe ama-carbohydrates, izinto ezine-nitrogen, usawoti we-inorganic, amavithamini namanzi, njll. Akuyona nje into eyisisekelo yokuhlinzeka ngokudla kwamangqamuzana nokukhuthaza ukwanda kwamangqamuzana, kodwa futhi nendawo yokuhlala yokukhula nokukhiqizwa kwamangqamuzana. .Indawo yokugcina okuphakathi ingu-2°C-8°C, ivikelekile ekukhanyeni.

3. Ama-reagents ahlukahlukene: Ngaphezu kokugcinwa kwe-serum ne-culture medium, amabhodlela aphakathi kwe-PETG angasetshenziswa njengeziqukathi zokugcina ama-reagents ahlukahlukene ezinto eziphilayo, njengama-buffers, ama-dissociation reagents, ama-antibiotics, izixazululo ze-cell cryopreservation, izixazululo zombala, izithasiselo zokukhula, njll. Amanye alawa ma-reagents adinga ukugcinwa ku--20 ° C, kanti amanye agcinwe ekamelweni lokushisa.Kungakhathaliseki ukuthi iyiphi indawo ezungezile, ibhodlela eliphakathi lingahlangabezana nezidingo zabo zokulondoloza.

I PETG medium ibhodlela ngokuyinhloko esetshenziswa ukubamba ngenhla izixazululo ezintathu.Ukuze kube lula ukubhekwa okubonakalayo komthamo wesixazululo, kunesilinganiso emzimbeni webhodlela.Izixazululo ezingenhla zisetshenziswa ngokuyisisekelo kusiko lamaseli, futhi kufanele kuqashelwe ekusebenzeni kwe-aseptic lapho wengeza.