Ziningi izingcezu zamangqamuzana ngemva kokulungiswa komshini kokumiswa kweseli.Ungazisusa kanjani lezi zingcezu?Ake sibheke izindlela ezahlukahlukene:

1. Sebenzisa indlela yokuhlanza.Lapho amaseli ehlanjululwa, angaphinde ande, abe maningi kakhulu, futhi imfucumfucu yamangqamuzana izoba kancane kancane.
2. Kukhona futhi ukuhlaliswa kwemvelo.Amaseli ahlala ngokushesha kunezingcezu eziningi: : Hambisa ukumiswa kweseli kui-centrifuge tube, futhi lapho amangqamuzana amaningi ecwila, isixazululo esingenhla singamuncwa, bese sengezwa kwisisombululo sesiko sokumisa amaseli.Le ndlela ingasetshenziswa ngokuphindaphindiwe, kuyilapho isikhathi ngasinye ungabheka ukuthi iyahlangabezana yini nezidingo zakho ngaphansi kwesibonakhulu.
3. I-centrifuge enesivinini esiphansiingasusa udoti, ngokuvamile 700g, 5mins
4. Lapho i-centrifuging, ngaphansi kwesimo sokuthi inani lamaseli lanele, nciphisa isikhathi se-centrifugation, njenge-5min, 1000rpm, esikhundleni se-3min, 1000rpm, futhi ususe amandla angaphezu kwamandla, ngoba i-necrosis kanye nemfucumfucu ngokuvamile ku-supernatant!Lo msebenzi udinga ukwenziwa kanye kuphela phakathi nenqubo yokuwasha ngaphambi kokufukamela!

Nakuba ziningi izindlela zokususa imfucumfucu yamaseli, uzothola ukuthi i-centrifugation ingenye yezindlela ezisebenza kahle kakhulu!

Futhi ngenxa yokuthi izicubu zezitshalo namaseli anezindonga zamaseli ezakhiwe i-cellulose, i-hemicellulose ne-pectin, ngokuvamile kuyadingeka ukugaya isihlabathi se-quartz noma impushana yengilazi ngesixazululo esifanelekile sokukhipha noma ukwelapha nge-cellulase ukuze ufeze inhloso.Ukuhlukana kwamangqamuzana amagciwane kunzima kakhulu, ngoba wonke uhlaka lwamathambo e-bacterial cell wall empeleni luyisibopho esihlangene sama-peptidoglycan cystic macromolecules, aqinile kakhulu.Izindlela ezivamile zokuphula izindonga zamangqamuzana ebhaktheriya zihlanganisa ukuchotshozwa kwe-ultrasonic, ukugaya isihlabathi, i-high pressure extrusion noma ukwelashwa kwe-lysozyme.Ngemva kokuba izicubu namaseli ephukile, isigcinalwazi esifanele siyakhethwa ukuze kukhishwe amaprotheni afunekayo.Izinto ezingancibiliki njengezicucu zamaseli zikhishwa ngawoi-centrifugenoma ukuhlunga.

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