I-Fluorescence Quantitative PCR Detection 96 amasampula
Njengenketho edingekayo yokuhlaziywa komthamo webhayoloji yamangqamuzana, uhlelo lwe-PCR lwesikhathi sangempela lusetshenziswe kabanzi emikhakheni ehlukahlukene efana nocwaningo lwesayensi, ukutholwa komtholampilo nokuxilongwa, ikhwalithi nokuhlolwa kokuphepha, kanye nezicelo zesayensi.
Isistimu ye-PCR Yesikhathi Sangempela
Iqiniso 96
Izici
• Kufika kumashaneli angu-6 okubona i-fluorescence evumela i-multiplex PCR.
• Yehlisa ngempumelelo i-crosstalk enemibala eminingi kanye nomphumela onqenqemeni, akukho ukulungiswa kwe-ROX okudingekayo ukuze kuncishiswe isampula nokusetshenziswa kwe-reagent
• Indlela yokuskena emisha kanye nobuchwepheshe bokuhlukaniswa kwesignali okuxazululwe yisikhathi ukuze kuthuthukiswe ukuzwela kokutholwa
• Ubuchwepheshe obuhlukile bokunxeshezelwa kwezinga lokushisa ukuze kuncishiswe "umphumela onqenqemeni"
• Isofthiwe esebenziseka kalula
• Ubuchwepheshe obusha obunokukhanya kwe-LED okuhlala isikhathi eside bunikeza imiphumela yokwethembeka
Imingcele Yezobuchwepheshe
| Isistimu yokulawula izinga lokushisa | |
| Umthamo wesampula | 96 |
| Ivolumu yokusabela | 10-50 μl |
| Ubuchwepheshe bomjikelezo oshisayo | I-Peltier |
| Ubukhulu.Izinga lokushisa/Ukupholisa | 6.0° C/s |
| Ibanga lokushisa lokushisa | 4 – 100 °C |
| Ukunemba kwezinga lokushisa | ± 0.2°C |
| Izinga lokushisa elifanayo | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
| Ibanga lokusetha igrediyethi yezinga lokushisa | 30–100°C |
| Ibanga lokusetha umehluko wegrediyethi yezinga lokushisa | 1 – 36°C |
| Isistimu yokutholwa | |
| Umthombo wokukhanya ojabulisayo | 4/6 monochrome ama-LED asebenza kahle kakhulu |
| Idivayisi yokuthola | I-PMT |
| Imodi yokutholwa | Ubuchwepheshe bokuhlukanisa isignali exazululwe yisikhathi |
| Ububanzi be-wavelength obujabulisayo/wokubona | 455-650nm/510-715nm |
| Iziteshi ze-Fluorescent | 4/6 iziteshi |
| Udayi osekelwe | I-FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Ukuzwela | Ikhophi yofuzo olulodwa |
| Isixazululo | 1.33 umehluko wenombolo yekhophi ungahlukaniswa nge-single-plex qPCR |
| Ibanga elinamandla | Ama-oda ayi-10 wamakhophi wobukhulu |
