Ungakhetha kanjani izinto ezisetshenziswayo zesiko lamaseli?
1. Thola indlela yokulima
Ngokwezindlela ezihlukene zokukhula, amaseli ahlukaniswe abe izigaba ezimbili: amaseli anamathelayo namaseli amisiwe, futhi kukhona namaseli angakhula kukho kokubili okunamathelayo nokumisiwe, njengamaseli e-SF9.Amaseli ahlukene anezidingo ezihlukene zezinto ezisetshenziswayo zesiko lamaseli.Amaseli anamathelayo ngokuvamile asebenzisa izinto ezisebenzisekayo ezilashwe i-TC, kuyilapho amaseli amisiwe engenazo izidingo ezinjalo, kodwa okusebenzisekayo okulashwe nge-TC nakho kulungele ukukhula kwamaseli amisiwe.Ukukhetha izinto ezisetshenziswayo ezifanele, indlela yesiko lamaseli kufanele kuqala inqunywe ngokuya ngohlobo lweseli.
2. Khetha uhlobo lwezinto ezidlekayo
Izinto ezisetshenziswayo ze-cell culture zihlanganisa amapuleti e-cell culture, izitsha ze-cell culture, ama-flask square square, ibhodlela le-cell roller, izimboni zamaseli,Amapayipi e-serological, njll. Lezi zinto ezisebenzisekayo zinezici zazo ngokwendawo yesiko, indlela yokusetshenziswa, kanye nesakhiwo sayo yonke.Ibhodlela lesiko liyisiko elivaliwe, elinganciphisa ukungcola;isiko plate kanyepetri dishkuyisiko elivulekile kancane, elilungele ukuhlolwa kokulawula kanye nokuhlolwa kwe-gradient, kodwa futhi kungenzeka ukuthi kubangele ukungcoliswa kwamagciwane, okudinga ama-opharetha aphezulu.Ezinye izinto ezisetshenziswayo nazo zidinga ukusetshenzwa ngemishini ekhethekile.Isibonelo, i-cell shaker idinga ukusebenzisa ukudlidliza kwe-shaker ukuze yenze amaseli axhumane kangcono nomoya, futhi imboni yamaseli enezendlalelo ezingu-40 idinga okokusebenza okuzenzakalelayo.Ngamafuphi, lapho kukhethwa uhlobo lwezinto ezisetshenziswayo, kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezidingo zokuhlola kanye nezintandokazi zokusebenza komuntu siqu.
1.Multi-kahlecell isiko Plates: Amafomethi wesiko lamaseli asebenzisa amapuleti amasiko amaseli anemithombo eminingi athola ukuduma ngoba enza kube lula ukucwaninga okuguquguqukayo okuningi, anciphisa isikhathi sokuhlola, futhi onge ama-reagents abizayo.Ukwengeza kuma-micro-plates asezingeni eliphezulu, ama-micro-plates akhethekile athuthukisiwe ukuze kube lula i-3D kanye nesiko lamaseli e-organotypic.
1) Inani lemigodi
Kuya ngezinga elidingekayo le-flux, kanye noma ngaphandle kosizo lomshini.6, 12, 24 kanye namanye amapuleti asebenza kahle amaseli angangezwa ngesandla.Ku-96 - kahleamapuleti amasiko amaseli, kungcono ukuba nosizo lwe-pipette kagesi noma umshini.
2) Ukuma kwembobo
Iphansi lomthombo lingakhethwa ukuthi libe isicaba (F-bottom), eliyindilinga (U-bottom), noma libe yi-tapered, kuye ngohlobo lweseli kanye nesicelo esingezansi.
3) Umbala wepuleti
Umbala we-perforated plate nawo uhlobene eduze nesicelo.Uma amaseli ebhekwa ngesibonakhulu sokungafani kwesigaba noma ngeso lenyama, ipuleti elibonisa ngale elinemithombo eminingi yamaseli lingakhethwa.Kodwa-ke, izinhlelo zokusebenza ezingaphandle kokukhanya kokukhanya okubonakalayo (okufana ne-luminescence noma i-fluorescence), ezinemibala eminingiamapuleti amasiko amaseli(okufana nomhlophe noma omnyama) kuyadingeka.
4) Ukwelashwa kwendawo
Iyiphi indlela yokwelapha engaphezulu kwamaseli ongayikhetha incike ekutheni uhlakulela ukumiswa noma amaseli anamathelayo.
2.Ama-flask amasiko eseli: Indawo yesiko isuka ku-25-225 cm², futhi ngokuvamile ilungiswa phezulu, ilungele ukunamathela kwamaseli nokukhula.225cm² kanye 175cm²ama-cell culture flasksasetshenziselwa kakhulu isiko lezinga elikhulu (elifana nesiko lamaseli e-monoclonal, njll.), 75cm² isetshenziswa kakhulu ekuhloleni amaseli okuvamile (indima evamile, ukulondolozwa kwamaseli, amaseli okuhlolwa, njll.), 25cm² ngokuvamile isetshenziselwa Ingakwazi zisetshenziselwe ukuvuselela amaseli noma isiko lapho kunamaseli ambalwa, futhi lapho kwenziwa amangqamuzana ayinhloko, amabhodlela amaningi angasetshenziswa ukugwema ukungcola.
3.I-Erlenmeyer flask: Uma kuqhathaniswa nezinto ezisetshenziswayo ezifana nezimboni zamaseli kanye nebhodlela le-cell roller, inendawo encane yesiko lamaseli futhi iyithuluzi lezomnotho lamaseli.Umzimba webhodlela weflask wenziwe nge-polycarbonate (PC) noma i-PETG impahla.Umklamo oyingqayizivele we-triangular shape wenza kube lula ukuthi i-pipette noma i-cell scraper ifinyelele ekhoneni lebhodlela, okwenza ukusebenza kwesiko lamaseli kube lula kakhulu.Ii-erlenmeyer flaskI-cap yenziwe ngezinto eziphezulu ze-HDPE, ezihlukaniswe zibe yi-cap yokuvala kanye ne-cap ephefumulayo.I-cap yokuvala isetshenziselwa isiko elivaliwe legesi noketshezi.Ikepisi eliphefumulayo lifakwe ulwelwesi lwesihlungi se-hydrophobic phezulu kwekepisi yebhodlela.Ivimbela ukungena nokuphuma kwama-microorganisms, ivimbela ukungcola, futhi iqinisekise ukushintshaniswa kwegesi, ukuze amaseli noma amagciwane akhule kahle.
Osayizi abajwayelekile bokuzamazama kwe-conicalama-flask e-erlenmeyer125ml, 250ml, 500ml, 1000ml kanye3L,5L amaflaski e-erlenmeyer asebenza kahle kakhulu, Ukuze ubuke umthamo we-medium futhi ubambe isimo sokukhula samaseli, isikali sizophrintwa emzimbeni webhodlela.Inqubo yeseli idinga ukwenziwa endaweni eyinyumba.Ngakho-ke, i-Erlenmeyer flask izophathwa ngokukhethekile inzalo ngaphambi kokuba isetshenziswe ukuze kuzuzwe umphumela wokungabi bikho kwe-DNase, i-RNase, kanye nezithako ezitholakala ezilwaneni, ezihlinzeka ngezimo ezinhle zokukhula kwamaseli.ezizungezile.
4.Izingqimba eziningiImboni Yeseli: Imboni yamaseli Ifanele ukukhiqizwa kwenqwaba yezimboni, njengemithi yokugoma, amasosha omzimba e-monoclonal noma imboni yezemithi, kodwa futhi nemisebenzi yaselabhorethri kanye nesiko lamaseli elikhulu.Elula futhi esebenzayo, gwema ngempumelelo ukungcola.Ifekthri Yeseli Enekhava Evaliwe: Isembozo asinazo izimbobo zokungenisa umoya, futhi sisetshenziswa kakhulu ngaphansi kwezimo ezingenaso isikhutha njengama-incubator kanye nezindlu zokugcina ukushisa.I-Cell Factory enekhava evaliwe ingavimbela ukuhlasela kwamagciwane angaphandle futhi isize ukudala indawo enhle yokukhula yokukhula kwamangqamuzana.Isembozo esiphefumulayo: Kunezimbobo zokungenisa umoya ngaphezulu kwekhava, ezisetshenziswa kakhulu endaweni ye-carbon dioxide.Izimbobo zokungenisa umoya zivumela i-carbon dioxide endaweni ukuthi ingene embonini yeseli, okwenza izimo zokukhula ezifanele zokukhula kwamaseli.Kukhona ungqimba olu-1, izendlalelo ezi-2, izendlalelo ezi-5, izendlalelo eziyi-10, izendlalelo ezingama-40izimboni zamaselietholakalayo.
5.Isiko lamaseliibhodlela le-roller: 2L & 5L Amabhodlela Roller Afanele izinhlobonhlobo ze-adherent cell cultures kanye namasiko e-Suspension cell, okuhlanganisa amaseli e-Vero, amaseli we-HEK 293, amaseli e-CAR-T, ama-MRC5, amaseli e-CEF, ama-porcine alveolar macrophages, amaseli e-myeloma, amaseli e-DF-1, Amaseli e-ST, amaseli e-PK15, amaseli e-Marc145 amanye amaseli anamathelayo.Ibuye ifanele isiko elimile lamaseli amisiwe njengamaseli e-CHO, amaseli ezinambuzane, amaseli e-BHK21 namaseli e-MDCK.
3.Khetha ukucaciswa kwezinto ezisetshenziswayo.
Ukuhlolwa kwesiko lamaseli esilinganiso esikhulu kudinga okusebenzisekayo okunendawo enkulu yesiko ukuze kusekelwe, kuyilapho izivivinyo ezincane zikhetha okusebenzisekayo ngendawo encane.Izimboni zamaseli zisetshenziselwa kakhulu isiko lamaseli amakhulu, njengokukhiqizwa kokugoma, amasosha omzimba e-monoclonal, imboni yezemithi, njll.;amapuleti amasiko, izitsha, namaflask afanele isiko lamaseli amancane ezindaweni zokucwaninga;ngaphezu ukumiswa cell isiko, the flask can futhi Ukuze medium ukulungiselela, ukuxuba kanye isitoreji.Ngokwesilinganiso se-cell culture, thola ukucaciswa okuqondile kwezinto ezisetshenziswayo.
Izinto ezisetshenziswayo ze-cell culture ziyisisekelo sokuqinisekisa ukukhula okuhle kwamaseli, futhi ziwukhiye wokusheshisa inqubo yokuhlola nokuqinisekisa umphumela wamasiko.Ekukhetheni, izici ezifana nendlela yesiko lamaseli, isikali samasiko, nezimo zaselabhorethri kufanele zicatshangelwe ngokuphelele.sidinga ukusebenzisa ezinye izinto ezisetshenziswayo lapho senza isiko lamaseli, isibonelo,Isiphathi se-CellDisk&umthwali oyindilinga we-CellDisk,amathiphu we-pipette,ifilimu yokuvala,ama-pipettes, njll., i-Luoron nayo ingahlinzeka.
I-LuoRon Biotech Co., Ltd igxile ocwaningweni, ekuthuthukisweni, ekuthengisweni nasekusezweni kwezinto ezisetshenziswayo zebhayoloji.Imboni yokukhiqiza inendawo yezitshalo engamamitha-skwele ayi-10,000.Ine-workshop yokukhiqiza ehlanzekile yebanga le-100,000, ishabhu yokuhlangana yezinga le-10,000 kanye neshabhu yokusebenzela yokucwaninga ngesikhunta enembayo kanye nokukhiqiza.
Ngamafuphi, lapho kukhethwa uhlobo lwezinto ezisetshenziswayo, kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezidingo zokuhlola kanye nezintandokazi zokusebenza komuntu siqu.Vele, kubalulekile ngokulinganayo ukukhetha iplatifomu efana ne-LuoRon enemikhiqizo yekhwalithi ephezulu nehlukahlukene, ukunikezwa okuzinzile, ikhwalithi eqinisekisiwe kanye nenkonzo.I-LuoRon ingahlinzeka ngohlu olugcwele lwezinsizakalo zokuthenga okukodwa kwezinsiza zocwaningo lwesayensi zamalabhorethri emkhakheni wesayensi yempilo yomhlaba wonke, imboni yezokwelapha, ukuvikelwa kwemvelo, ukuphepha kokudla, izinhlangano zikahulumeni, kanye nemithi yokwelapha.
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