I-Spherical Bioreactor Microcarrier

CellDeks1umthwali oyindilingaiyindilinga encane ethwalayo eyakhiwe ngokubopha i-diethylaminochloroethane kuma-agarose micro sphere, okwenza amangqamuzana axhunywe kalula., esetshenziselwa kakhulu ucwaningo nokuthuthukiswa nokukhiqizwa kwemikhiqizo yebhayoloji.I-lt ilungele i-subculture yamaseli e-Vero, amaseli e-HEK293, amaseli e-Mrc-5, amaseli e-CHO, amaseli e-BHK21, amaseli e-MDCK, amaseli e-ST, amaseli e-Marc145, amaseli e-SF9/21, njll. amaseli e-myeloma kanye nesiko lamaseli ayisisekelo njengamaseli e-CAR-T.

Zombili izithwali zingasetshenziselwa isiko lamaseli anamathelayo.Ukuze kuzuze umnotho, amagciwane atholakala ngokuqhubekayo, njenge-rabies virus, i-swine fever virus, esebenzisa ama-flake carriers;Amanye amagciwane asebenzisa ama-vector ayindilinga.Ukusetshenziswa kwezithwali ze-flake noma abathwali abayindilinga kunqunywa ngokuya ngemishini yabo kanye nenqubo

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

● Izici zomkhiqizo

Ukuhlukaniswa okuphumelelayo nokulula kwamasiko namaseli, ukuvunwa kwemikhiqizo, ukugcotshwa noma ukuphakelwa okuqhubekayo.
.Ingatshalwa kuma-bioreactors amakhulu avuselelwe, ama-bioreactor asetshenziswa kanye kanye namaflask okunyakazisa ukuze kunikeze indawo eyanele yokukhula kwamaseli.
Kulula ukukhulisa amaseli.Amaseli angagaywa kulowo ophethe bese ejova esiphathini esisha, noma isithwali esisha singengezwa ngokuqondile ukuze sibone indlela "yebhola-kuya-ibhola" yokwandisa isiko.

● Imingcele Eyinhloko Yomkhiqizo

Ikhodi ye-resin	I-Microcarrier Celldex1
Ukubukeka	izinhlayiya ezimhlophe, Ayinaphunga futhi ayinambitheki
Ukuminyana * (g/ml)	<1.045
Usayizi we-particle (pm)	Okomile: 50-100In saline:145-240
Umthamo wokushintshaniswa (mmol / g owomile)	1.4-1.6
Isici sokuvuvukala* (ml/g isisindo esomile)	17-22
Isivinini se-Sedimentation	10-12cm/ min
Ukulahlekelwa ekomisweni	< 10%
Okuqukethwe kwe-Microbial (inani lamakholoni/g isisindo esomile)	< 100
Igramu ngayinye yesisindo esomile iqukethe cishe inani lama-microcarriers	4.3x106
Cishe.indawo (emanzi	4500cm2
Isicelo	Ifanele isiko lokumiswa kwamaseli ancike ku-anchorage, asetshenziswa ekukhiqizeni imigomo namaprotheni, njll.

I-Photomicrograph yamaseli e-Vero akhuliswe ku-CellDex1 amahora angama-24

Isithombe samaseli e-Vero akhuliswe ku-CellDex1 isikhathi esingu-48h (320 x)

Isithombe samaseli e-Vero akhuliswe ku-CellDex1 ama-72h (320 x)

● Ipharamitha Yomkhiqizo

Inombolo Yomkhiqizo	Ukucaciswa	Pakishaiminyaka yobudala	Ibhodlela/ikesi
C100050	Ideskithophu Yeseli1	50/ibhodlela	40
C100250	Ideskithophu Yeseli1	250/ibhodlela	20
C100500	Ideskithophu Yeseli1	500/ibhodlela	10
C100001	Ideskithophu Yeseli1	1 kg/ibhodlela	4
C100005	Ideskithophu Yeseli1	5 kg/ibhodlela	1