Imboni yamaseli iyinto evamile esetshenziswa esikalini esikhulu samaseli, esetshenziselwa ikakhulukazi isiko lamaseli abambelelayo.Ukukhula kwamangqamuzana kudinga zonke izinhlobo zemisoco, ngakho ziyini?
1. Isikhulumi samasiko
I-cell culture medium inikeza amaseli embonini yamaseli imisoco edingekayo ukuze akhule, okuhlanganisa amacarbohydrates, ama-amino acid, usawoti wezinto ezingaphili, amavithamini, njll. Kunezinhlobonhlobo zemidiya yokwenziwa etholakalayo yezidingo zokudla okunomsoco zamangqamuzana ahlukene, njenge-EBSS. , Ukhozi, MEM, RPMll640, DEM, njll.

2. Ezinye izithako ezengeziwe
Ngaphezu kwezakhi eziyisisekelo ezinikezwa imidiya ehlukahlukene yokwenziwa, ezinye izakhi, njenge-serum nezici, zidinga ukungezwa ngokuvumelana namaseli ahlukene kanye nezinjongo ezihlukahlukene zesiko.
I-Serum inikeza izinto ezibalulekile ezifana ne-extracellular matrix, izici zokukhula kanye ne-transferrin, kanye ne-fetal bovine serum ivame ukusetshenziswa.Ingxenye ye-serum ezongezwa incike kuseli kanye nenjongo yocwaningo.I-10% ~ 20% i-serum ingagcina ukukhula okusheshayo nokwanda kwamangqamuzana, eyaziwa ngokuthi indawo yokukhula;Ukuze kugcinwe ukukhula okuhamba kancane noma ukungafi kwamaseli, i-serum engu-2% ~ 5% ingangezwa, ebizwa ngokuthi isiko lokulondoloza.
I-Glutamine iwumthombo we-nitrogen obalulekile wokukhula kwamangqamuzana futhi idlala indima ebalulekile enqubweni yokukhula kwamaseli kanye ne-metabolism.Nokho, ngenxa yokuthi i-glutamine ayizinzile kakhulu futhi kulula ukuyehlisa esixazululweni, ingabola cishe ngo-50% ngemva kwezinsuku ezingu-7 ku-4℃, ngakho i-glutamine idinga ukungezwa ngaphambi kokusetshenziswa.
Ngokuvamile, imidiya ehlukahlukene kanye ne-serum isetshenziselwa isiko lamaseli, kodwa ukuze kuvinjelwe ukungcoliswa kwamangqamuzana phakathi nesiko, inani elithile lemithi elwa namagciwane, njenge-penicillin, i-streptomycin, i-gentamicin, njll, nayo yengezwa kwabezindaba.