Shake flask culturisesigabeni sokuhlolwa kobunzima kanye nesiko (ukuhlolwa komshayeli), izimo zesiko kufanele zibe eduze ngangokunokwenzeka nezimo zesiko lokukhiqiza ukuvutshelwa, umthwalo wokusebenza mkhulu, isikhathi eside, ukusebenza okuyinkimbinkimbi.Izici ezithinta ukusebenza kahle okuphezulu kwesiko le-flask shaking ikakhulukazi izinga lokushisa kwamasiko, i-amplitude enyakazayo ye-shaker, inani le-flask enyakazayo, i-pH yendawo yesiko, i-viscosity ye-medium, njll. Izinga lokushisa lamasiko: izinga lokushisa lokukhula kwe-mycelium Isikhunta esidliwayo esihlukile sihlukile futhi, izinga lokushisa elifanele lokukhula liphakathi kuka-22 ℃ no-30 ℃, uma izinga lokushisa lesiko liphansi kakhulu, ukukhula kwe-mycelium kuhamba kancane;Lapho izinga lokushisa liphezulu kakhulu, ama-pellets e-mycelium ayexegekile futhi enqabile, futhi amandla kanye nekhwalithi yamapellets e-mycelium ayehla.

Ukunyakaziswa kwemvamisa nokusebenza kahle okuphezulu kokulayisha ibhodlela lokunyakazisa: isikhunta esidliwayo siyisikhunta se-aerobic, isiko le-liquid, ikakhulukazi ngokumuncwa komoyampilo oncibilikisiwe endaweni yamasiko.I-oxygen encibilikile endaweni yesiko ithinteka kakhulu yi-viscosity ye-medium, inani loketshezi esitsheni, imvamisa ye-oscillation nezinye izici.Imvamisa yokuthuthumela inkulu, i-flask enyakazayo incane, ukugxila kwe-medium kuya phezulu, i-oxygen encibilikisiwe ye-medium iphezulu, kanti enye indlela iphansi.Ngokuvamile isivinini se-rotary shaker singu-180-220 RPM/min, ukuphindaphinda kungu-80-120 RPM/min, amplitude 6-7cm.

I-Ph of Culture medium: I-PH ye-culture medium ithinta ngokuqondile ukumuncwa kwezakhi, umsebenzi we-enzyme kanye nokukhula kwe-mycelial pellet.I-pH ecacile kufanele ilungiswe ngaphambi kokuvalwa inzalo, isikhunta esidliwayo esiningi ku-pH 2.0-6.0.Ukuze uvimbele ukuguquka okukhulu kwe-PH endaweni yesiko, i-calcium carbonate, i-phosphate nezinye izinto ze-buffer zivame ukungezwa endaweni yesiko.

I-viscosity ephakathi: I-viscosity ephakathi ithinta ngokuqondile inani le-oxygen encibilikisiwe kuyo, futhi ithinta ukwakheka kwamapellets e-mycelial.Imiphumela yabonisa ukuthi lapho i-viscosity ye-culture medium ikhula, ububanzi be-mycelium pellets behla, inani landa, futhi isivuno sakhula.Ngakho-ke, ngokuya ngezidingo ze-liquid strains kububanzi bama-pellets e-mycelium, indawo yesiko ene-viscosity ethile kufanele imiswe.Isiko lamaseli kuwumsebenzi onzima, ikakhulukazi uma udinga ukukhuliswa ngosizo lwe-shaker, njenge-shaker esebenza kahle kakhulu, kufanele kubhekwe kabanzi, ukuze kuqinisekiswe ukuqhubeka kahle kwesiko lamaseli.