Namuhla, ngokuthuthuka okusheshayo kwesayensi nobuchwepheshe,ubuchwepheshe be-cell cultureisetshenziswa kakhulu emikhakheni eyahlukene yocwaningo lwesayensi efana ne-cytology, i-immunology, kanye nemithi yokuhlola ukusiza ucwaningo nokuthuthukiswa kwemithi emisha yokugoma nemithi elwa nesimila.Isiko lamaseliidinga indawo ethile.Ngakho-ke, izinto ezisetshenziswayo zesiko lamaseli, okuyiziqukathi ezidingekayo, ngokuvamile zinezici ezilandelayo:

1. Ukucaca okuphezulu: Izinto ezisetshenziswayo ezijwayelekile zeizinto ezisetshenziswa amaselizihlanganisa i-PC, i-PS (i-polystyrene), i-PETG, njll. Lezi zinto zinezinga eliphezulu lokubonisa obala.Yenza izinguquko ezihambisanayo.

2. Ayikho i-DNase/RNase: Ukukhula kweseli kunezidingo eziphakeme uma kuqhathaniswa ngendawo ezungezile, futhi kudinga ukwenziwa ngaphansi kwezimo ezifanele ezifana nezinga lokushisa, ingcindezi ye-osmotic, ne-pH.Phakathi kwabo, ukungabi khona kwe-DNase ne-RNase kusho ukuthiizinto ezisetshenziswa amaselibahlinzwe inzalo ekhethekile.Ukwelashwa kuqeda izici ezihlukahlukene ezivimbela ukukhula kwamangqamuzana.Ukwenziwa inzalo kwe-electron beam irradiation kuyindlela evame ukusetshenziswa yokuvala inzalo emikhiqizweni esebenzisekayo.

3. Ukwelashwa okungaphezulu: Amaseli amaningi ezilwane adinga ukuxhunywa kusixhumanisi esibonakalayo sesigaba esiqinile ukuze akhule, futhi isixhumi esibonakalayo sesigaba esiqinile se-hydrophilic kuphela esinganamathisela amaseli.Njengamanje, iningi lamasiko amaseli emakethe angamaseli abambelelayo.Ukuze kwandiswe ukusebenza kokunamathela kobuso bezinto ezisetshenziswayo zamaseli futhi zifanele ukukhula kokunamathiselwe kweseli, lezi zinto ezisebenzisekayo zizobhekana nokwelashwa okukhethekile kokulungiswa kwendawo ukuze kuthuthukiswe i-hydrophilicity yazo.Izinto ezisetshenziswa amaselingemva kokuguqulwa kwendawo ngokuvamile afaneleka ukutshalwa kwamaseli amisiwe.

Imikhiqizo ehlangabezana nesidingo semakethe inokuncintisana kakhulu.Ukucaca okuphezulu, i-DNase/i-RNase-free, nokuguqulwa kwendawo ezinye zezici eziyisisekelo zezinto ezisetshenziswayo zamaseli.Lezi zici zibuye zikhuthaze ukukhula okungcono kwamangqamuzana nokuzala.

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