Amabhodlela amasiko amaselizivame ukusetshenziswa emasikweni amaseli abambelelayo, lapho amaseli kufanele axhunywe ngaphezu kwento esekelayo ukuze akhule.Manje-ke yini ekhangayo phakathi kweseli elinamathelayo kanye nento esekelayo, futhi iyiphi indlela yeseli elinamathelayo?

Ukunamathela kweseli kusho inqubo yamaseli ancike ekunamatheleni anamathisele futhi asakazeke endaweni yesiko.Ukuthi iseli lingaxhunywa endaweni yesiko kuncike ezicini zeseli ngokwalo, emathubeni okuxhumana phakathi kweseli nendawo yesiko, nasekuhambisaneni phakathi kweseli nendawo yesiko, ehlobene nekhemikhali kanye nemvelo. izakhiwo ezingokomzimba ebusweni.

Izinga lokunamathela kwamaseli nalo lihlobene nezakhi zamakhemikhali nezomzimba zendawo yesiko, ikakhulukazi ukuminyana kweshaja endaweni yesiko.I-colder ne-fibronectin ku-serum ingakwazi ukuhlanganisa indawo yesiko kuseli, okuzuzisa ukusheshisa izinga lokunamathela kwamaseli.Ngaphezu kwalezi zici ezingenhla, ukusabalala kwamaseli endaweni yesiko nakho kuhlobene nesimo esingaphezulu, ikakhulukazi ubushelelezi.

Amangqamuzana amaningi ezilwane ezincelisayo akhula ku-vivo kanye ne-in vitro enamathiselwe kuma-substrates athile, okungaba yi-vitro okungamanye amaseli, i-collagen, amapulasitiki, njll. Amaseli aqala akhiqiza i-matrix engaphandle, enamathela ebusweni be-vial yesiko lamaseli.Iseli libe selibophezela kulawa matrices angaphandle kwamaseli ngokusebenzisa izici zokunamathela ezivezwe endaweni yayo.

Ukwengeza, ukuze kuthuthukiswe kangcono ukubambelela kwamaseli, indawo yokukhula yebhodlela le-cell culture izophathwa ngokukhethekile ukwethula uquqaba lwama-hydrophilic, olusiza ukukhula kwamaseli abambelelayo.