Ibhodlela eliphakathi le-PETGiyisitsha sokugcina sepulasitiki esisobala esisetshenziselwa ukugcina i-serum, medium, buffer nezinye izixazululo.Ukuze ugweme ukungcoliswa kwamagciwane okubangelwa ukupakishwa, wonke afakwa inzalo, futhi le phakheji ivalwa inzalo yi-cobalt 60.

Ukuvala inzalo kusho ukususa noma ukubulala wonke amagciwane, amagciwane, isikhunta nezinye izinambuzane ebhodleleni eliphakathi le-PETG ngezindlela ezihlukahlukene zomzimba namakhemikhali, ukuze lifinyelele izinga lesiqinisekiso se-asepsis lika-10-6, okungukuthi, ukuqinisekisa ukuthi amathuba okusinda. ama-microorganisms esihlokweni esisodwa kuphela esigidini.Kungale ndlela kuphela lapho ama-microorganisms asephaketheni angavinjelwa ukuba abangele ukungcoliswa okwengeziwe kokuqukethwe kwangaphakathi.

I-Cobalt-60 inzalo ukusetshenziswa kwe-60Co γ-ray irradiation, esebenza kuma-microorganisms, ibhubhisa ngokuqondile noma ngokungaqondile i-nucleus ye-microorganisms, ngaleyo ndlela ibulale ama-microorganisms, idlala indima yokubulala amagciwane kanye nokuvalwa inzalo.Iwuhlobo lobuchwepheshe bokuvala inzalo ngokufakwa kwemisebe.I-γ-ray ekhiqizwa i-isotope cobalt-60 enemisebe ikhanyisa ukudla okupakishiwe.Enqubweni yokudlulisa amandla nokudluliswa kwamandla, imiphumela enamandla ngokomzimba nangokwebhayoloji ikhiqizwa ukufeza inhloso yokubulala izinambuzane, amagciwane abulala inzalo kanye nokuvimbela izinqubo zomzimba.I-60Co-γ-ray irradiation inzalo ubuchwepheshe "bokucubungula okubandayo", inzalo ekamelweni lokushisa, amandla aphezulu e-γ-ray, ukungena okunamandla, ekubulaleni inzalo ngesikhathi esifanayo, ngeke kubangele ukwanda kwezinga lokushisa lezinto, eyaziwa nangokuthi indlela yokuvala inzalo ebandayo.