I-cell passage culture isho inqubo yokuhlukanisa isiko libe izingxenye ezincane futhi liphinde lifakwe komunye umkhumbi wamasiko (ibhodlela) ukuze kuthuthukiswe isiko.Ukusebenza okuphezulu kwe-cell shakerkuyinto evamile esetshenziswayo yesiko leseli lokumiswa, ngakho-ke ungasebenzisa kanjani i-cell shaker esebenza kahle kakhulu ukuze kudlule iseli?

Ngokwemvelo yawo, amaseli amisiwe awabambeleli, ngakho-ke awekho ama-enzyme adingekayo ukuze awasuse ebusweni be-shaker esebenza kahle kakhulu.Elabhorethri evamile, indlela eqondile kanye ne-centrifugal passage ivame ukusetshenziselwa ukuhambisa amaseli amisiwe.Lapho amangqamuzana ebhekwa ukuthi aminyene ngamaphesenti angu-80 kuya kwangu-90 (i-cell suspension iba phuzi), amangqamuzana asuke eselungele ukudlula.

Uma amaseli ekhula kahle ngaphansi kwesibonakhulu, kungasetshenziswa indlela eqondile.I-medium ku-high-efficiency shaking flaskyahlukaniswa ngokulinganayo yaba isitsha sempucuko esisha, kwase kwengezwa nendlela entsha.Ukuminyana kwamaseli kwaqashelwa ngosuku olulandelayo ukuze kunqunywe ukuthi uketshezi lwaludingeka yini.

Uma isimo seseli sisibi, kufanele kusetshenziswe indlela ye-centrifugal passage.Okokuqala, ukumiswa kweseli kudluliselwa ku-i-centrifuge tube, i-centrifuged at 1000rpm for 5 min, khona-ke i-supernatant iyalahlwa, i-cell precipitates ihlakazeka kahle, futhi amaseli aphinde amiswe okwesikhashana nge-fresh medium.Ekugcineni, inani elifanele lokumiswa kweseli limuncwa ngotshani, lifakwe endaweni entshaisiko ibhodlela, futhi inani elifanele le-medium entsha liyengezwa.Qhubeka uhlakulele.

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