Uma sisebenzisa okunye okusebenzisekayo kwesiko lamaseli, sihlala sihlangabezana nenkinga yokudlula kweseli.Namuhla, sizokwabelana nawe kafushane ukuthi ungawasebenzisa kanjani ama-shake flasks asezingeni eliphezulu ukuze kudlule iseli.Lapho sisebenzisa amaflask ashukumisayo asebenza kahle kakhulu(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) ukuze uthole indawo yeseli, kunezindlela ezimbili ongakhetha kuzo, njengokuqoqa amaseli ngokufaka i-centrifugation bese uwadlula, noma uwaqondise. iphaseji.

Indlela ye-Centrifugal passage:

(1) Dlulisa amaseli ku-high-efficiency shake flask kanye ne-culture medium kuya kushubhu ye-centrifuge ye-centrifugation.

(2) Lahla amandla angaphezu kwavamile, engeza indlela entsha yesiko ku- i-centrifuge tube futhii-pipetteukwakha ukumiswa kweseli.

(3) Bala futhi ugome kumaflaski amasha empucuko ngokulandelanayo.

Uma kwamukelwa indlela eqondile, vumela amaseli amisiwe azinze kancane kancane phansi kweflask ye-shake esebenza kahle kakhulu, umunce u-1/2~2/3 we-supernatant, bese i-pipette yakhe ukumiswa kweseli ngaphambi kokudlula.

Okufanele sikunake ngesikhathi sokusebenza ukuthi i-trypsin kufanele ifudumale kuqala, futhi izinga lokushisa licishe libe ngu-37°C.Ijubane le-centrifugation kufanele lifanele.Uma isivinini siphansi kakhulu, amaseli awakwazi ukuhlukaniswa ngokuphumelelayo.Uma isivinini se-centrifugation siphezulu kakhulu futhi isikhathi siside kakhulu, amaseli azompindwa, abangele umonakalo noma ngisho nokufa.Amaseli kufanele abhekwe njalo, futhi uma ukungcola kutholakala, kufanele kubhekwane nakho ngesikhathi.