Isibalo sabantu abangenwe yizifo kanye nokufa kwabantu emhlabeni siqhubekile nokukhuphuka selokhu kwaqala ubhubhane lwe-COVID-19.Kusukela ngoSepthemba 2021, inani lokufa kwabantu emhlabeni wonke abavela ku-COVID-19 ladlula ezigidini ezi-4.5, ngamacala angaphezu kwezigidi ezingama-222.
I-COVID-19 ibucayi, futhi asikwazi ukuphumula.Ukutholwa kusenesikhathi, ukubika kusenesikhathi, ukuhlukaniswa kusenesikhathi kanye nokwelashwa kusenesikhathi kuyadingeka ukuze kunqanyulwe ngokushesha indlela yokudlulisela igciwane.
Ngakho-ke ungalithola kanjani i-Novel Coronavirus Virus?
Ukutholwa kwe-COVID-19 nucleic acid ukuhlola nokuhlolwa kwamacala aqinisekisiwe e-COVID-19, izigameko ezisolwayo ze-COVID-19 kanye nabantu abangenazimpawu ze-asymptomatic ngokusebenzisa izindlela zaselabhorethri.
1. Indlela ye-PCR yesikhathi sangempela ye-Fluorescence
Indlela ye-PCR ibhekisela ekuphenduleni kwe-polymerase chain, okwandisa ngokumangalisayo amanani amancane e-DNA.Ukuze kutholwe inoveli iCoronavirus, njengoba inoveli iCoronavirus iyigciwane le-RNA, i-RNA yegciwane idinga ukuguqulwa ku-DNA ngaphambi kokutholwa kwe-PCR.
Umgomo wokutholwa kwe-fluorescence PCR uthi: ngokuqhubeka kwe-PCR, imikhiqizo yokusabela iyaqhubeka nokunqwabelana, futhi ukuqina kwesiginali ye-fluorescence nakho kukhuphuka ngokulinganayo.Ekugcineni, ijika le-fluorescence amplification latholwa ngokuqapha ukuguqulwa kwenani lomkhiqizo ngokushintsha kwamandla e-fluorescence.Lena indlela esetshenziswa kakhulu njengamanje yokuhlola inoveli yeCoronavirus nucleic acid.
Nokho, amagciwane e-RNA wonakala kalula uma engalondolozwanga kahle noma engalethwa ukuze ahlolwe kusenesikhathi.Ngakho-ke, ngemva kokuthola amasampula esiguli, kudingeka agcinwe ngendlela evamile futhi ahlolwe ngokushesha ngangokunokwenzeka.Uma kungenjalo, kungenzeka kuholele emiphumeleni yokuhlolwa enganembile.
Amashubhu esampula egciwane (Asetshenziselwa ukuqoqwa, ukuthuthwa nokugcinwa kwamasampula egciwane le-DNA/RNA.)
2. Indlela yokulandelana ye-probe anchored polymerization
Lokhu kuhlola kusebenzisa amathuluzi akhethekile ukuze kutholwe ukulandelana kwezakhi zofuzo ezithwalwa ama-nanospheres e-DNA ekulandelaneni kwamaslayidi.
Ukuzwela kwalokhu kuhlolwa kuphezulu, futhi akulula ukuphuthelwa ukuxilongwa, kodwa imiphumela ibuye ithinteke kalula yizici ezihlukahlukene futhi ayinembile.
3. Indlela ye-chip yokukhulisa i-Thermostatic
Umgomo wokutholwa usekelwe ekuhlanganiseni okuhambisanayo kwama-nucleic acid phakathi kokuthuthukiswa kwendlela yokuthola, engasetshenziselwa ukulinganisa ikhwalithi noma inani lama-nucleic acid emzimbeni wezinto eziphilayo.
4. Ukutholwa kwamasosha omzimba
Ama-antibody reagents asetshenziselwa ukuthola amasosha omzimba e-IgM noma e-IgG akhiqizwa umzimba womuntu ngemva kokuba igciwane lingene emzimbeni.Amasosha omzimba e-IgM avela ngaphambi kwesikhathi futhi amasosha omzimba e-IgG avela kamuva.
5. Indlela yegolide ye-Colloidal
Indlela yegolide ye-Colloidal iwukusebenzisa iphepha lokuhlola igolide le-colloidal ukuze kutholwe, okuvame ukushiwo okwamanje iphepha lokuhlola lokubona ngokushesha.Lolu hlobo lokuhlola lusemizuzwini eyi-10 ~ 15 noma ngokuvamile, lungathola umphumela wokutholwa.
6. I-Chemiluminescence yezinhlayiya kazibuthe
I-Chemiluminescence i-immunoassay ebucayi kakhulu engasetshenziswa ukucacisa i-antigenicity yezinto.Indlela ye-magnetic particle chemiluminescence isekelwe ekutholweni kwe-chemiluminescence, ingeza ama-nanoparticles kazibuthe, ukuze ukutholwa kube nokuzwela okuphezulu kanye nesivinini sokuthola ngokushesha.
I-COVID-19 nucleic acid test VS antibody, yikuphi ongakhetha?
Ukuhlolwa kwe-Nucleic acid kusewukuphela kokuhlolwa okusetshenziselwa ukuqinisekisa ukutheleleka kwe-Novel coronavirus. Ezimweni ezisolwayo zenoveli yokuhlola i-Coronavirus nucleic acid negative, ukuhlolwa kwe-antibody kungasetshenziswa njengenkomba yokuhlola eyengeziwe.
I-Novel Coronavirus(2019-nCoV) I-Nucleic Acid Detection Kit (Indlela ye-Fluorescence PCR), ukuhlanzwa kwe-Nucleic acid kwamasampula angama-32 kungaqedwa ngemizuzu engama-20.
I-Real-time Fluorescence Quantitative PCR Analyzer(amasampula ayi-16, amasampula angama-96)
Isikhathi sokuthumela: Sep-13-2021
