Kanye amaseli thina isiko in

imboni yamaselizingcolile, eziningi zazo kunzima ukuzibamba.Uma amaseli angcolile eyigugu futhi kunzima ukuwathola futhi, izindlela ezilandelayo zingasetshenziswa ukuwakhipha.

1. Sebenzisa ama-antibiotics

Ama-antibiotics asebenza kangcono ekubulaleni amagciwaneizimboni zamaseli.Imithi eyinhlanganisela iphumelela kakhulu kunemithi yodwa.Imithi yokuvimbela iphumelela kakhulu kunemithi ngemva kokutheleleka.Imithi yokuvimbela ngokuvamile isebenzisa ama-antibiotic amabili (penicillin 100u/mL plus streptomycin 100μg/mL).Ngemuva kokungcola, indlela yokuhlanza idinga ukuba ibe nkulu izikhathi ezi-5 kuya kweziyi-10 kunesamba esivamile.Umuthi kufanele usetshenziswe amahora angama-24 kuye kwangama-48 ngemuva kokufakwa, bese uthathelwa indawo yinqubo evamile.Uketshezi lwamasiko.Le ndlela ingase isebenze ezigabeni zokuqala zokungcola.Ngaphezu kwe-penicillin ne-streptomycin, ama-antibiotic asetshenziswayo angaphinda ahlanganise i-gentamicin, i-kanamycin, i-polymyxin, i-tetracycline, i-nystatin, njll. Okuvame ukusetshenziswa yi-400 kuya ku-800 μg/mL kanamycin noma i-200 μg/mL tetracycline.Indlela esetshenziswayo iyashintshwa njalo ezinsukwini ezi-2 kuye kwezingu-3 futhi idluliselwe esizukulwaneni esi-1 kuya kwezi-2 ukuze zelashwe.Eminyakeni yamuva nje, kubikwe ukuthi i-4-fluoro, i-2-hydroxyquinoline (Ciprofloxacin, Cip), i-Pleu-romutilin derivative (i-Pleu-romutilin derivative, BM-Cyclin2: BM-1 kanye ne-tetracycline derivative (BM-2)) Ama-antibiotics asetshenziswa. ngempumelelo ekubulaleni i-mycoplasma uma isetshenziswa iyodwa noma ihlangene.Lawa ma-antibiotic amathathu wonke alungiswa abe yizixazululo ezigxile ku-250X ku-PBS futhi agcinwe ku -20°C ukuze asetshenziswe kamuva.Ukugxila kokusetshenziswa kwe-Cip ngu-10 μg/mL, i-BM-1 ingu-10 μg/mL, kanti i-BM-2 ingu-5μg/mL.Lapho usebenzisa, qala ngokufisa isiko elingcolisiwe, engeza i-RPMI1640 isiko eliqukethe i-BM-1, bese ufisa isiko lamasiko ngemuva kwezinsuku ezi-3, engeza i-RPMI1640 isiko eliqukethe i-BM-2, nesiko izinsuku ezi-4, njalonjalo izinsuku ezi-3 ezilandelanayo. .imijikelezo, kuze kube yilapho kufakazelwa yi-33258 fluorescent staining microscopy ukuthi i-mycoplasma isusiwe, khona-ke i-culture medium evamile yengezwa isiko kanye nokudlula izikhathi ezingu-3-4.

2. Ukwelashwa kokushisisa

Ukufukamela isiko lezicubu ezingcolile ku-41°C amahora angu-18 kungabulala i-mycoplasma, kodwa kunemiphumela emibi kumaseli.Ngakho-ke, ukuhlolwa kokuqala kufanele kwenziwe ngaphambi kokwelashwa ukuhlola isikhathi sokushisa esingabulala i-mycoplasma ngezinga eliphezulu futhi sibe nomthelela omncane kumaseli.Le ndlela ngezinye izikhathi ayithembekile.Uma uphathwa ngezidakamizwa kuqala bese ushisisa ku-41°C, umphumela uzoba ngcono.

3. Sebenzisa i-serum eqondene ne-mycoplasma

Ukungcola kwe-Mycoplasma kungasuswa nge-5% ye-rabbit mycoplasma immune serum (i-hemagglutination titer 1:320 noma ngaphezulu).Ngenxa yokuthi i-antibody ethile ingavimbela ukukhula kwe-mycoplasma, iba yi-negative ezinsukwini eziyi-11 ngemuva kokwelashwa kwe-antiserum futhi ihlala inegethivu ezinyangeni ezi-5 kamuva.inegethivu.Nokho, le ndlela inzima kakhulu futhi ayilula futhi iyonga njengokusebenzisa ama-antibiotic.

4. Ezinye izindlela

Ngaphezu kwalezi zindlela ezibalulwe ngenhla zokususa ukungcola, kunezindlela zokujova kanye nezindlela zokuvala inzalo ezilwaneni, izindlela ze-macrophage phagocytosis, izindlela zokwengeza i-bromouracil kokungcolile.isiko amabhodlelabese uwakhanyisela ngokukhanya, nezindlela zokuhlunga, njll., kodwa zonke zihlupha kakhulu futhi azisebenzi.Ngakho-ke, uma ukungcoliswa kwe-mycoplasma kwenzeka, ngaphandle uma kunenani elibaluleke kakhulu, ngokuvamile liyalahlwa futhi livuselelwe kabusha.

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