Ku-suspension cell culture,i-cell shake flaskluhlobo lwesiko lamaseli olusebenzisekayo.Ukukhula kwamaseli amisiwe kwakungancikile ebusweni bezinto ezisekelayo futhi akhula esimweni sokumiswa endaweni yesiko.Sinquma kanjani inani loketshezi okufanele lwengezwe esikweni langempela?

Imininingwane ejwayelekile ye-cell shaker ihlanganisa u-125ml, 250ml, 500ml kanye no-1000ml ukuze kuhlangatshezwane nezidingo zosayizi abahlukene besiko lamaseli.Isibonelo, amabhodlela angu-125ml kanye no-250ml anamandla amancane asetshenziswa kakhulu ekuhloleni okuncane, kuyilapho ukucaciswa kwe-500ml kanye ne-1000ml kusetshenziselwa ukuhlolwa kwesiko lamaseli esezingeni eliphakathi.Uma usebenzisa lolu hlobo lwezinto ezisetshenziswayo, ukudlidliza kwe-shaker kufanele kusetshenziselwe ukunciphisa izinga lokuhlangana kwamaseli nokugcina isimo esihle sokukhula samaseli.Inqubo yeseli idinga ukwenziwa endaweni eyinyumba.Ngakho-ke, i-flask yesiko likanxantathu izokwenziwa inzalo ngokukhethekile ngaphambi kokuba isetshenziswe ukuze kuzuzwe umphumela wokungabi bikho kwe-DNase, i-enzyme ye-RNA, kanye nezingxenye ezithathwe ezilwaneni, okuhlinzeka ngendawo enhle yokukhula kwamangqamuzana.

Ngokusho kokucaciswa okuhlukile kwebhodlela, ivolumu yokugcwalisa enconyiwe yezicaciso ezine zebhodlela ukusuka phansi kuye phezulu yi-30ml, 60ml, 125ml, 500ml.Ngokuvamile, umthamo wesixazululo kusiko lamaseli ulawulwa cishe ku-20% -30% wevolumu ephelele yebhodlela elinyakaziswayo, futhi kukhona umugqa ocacile wesikali emzimbeni webhodlela ukwenza lula ukubonwa komthamo wesixazululo. .

Okungenhla inani elinconyiwe loketshezi olungezwe emibhalweni ehlukene ye-cell shaker, engalungisiwe.Umthamo kufanele ucatshangelwe ngokugcwele ngokuya nezici zokukhula kwamangqamuzana kanye nokuminyana kwe-inoculation, ukuze kugwenywe umthelela wokukhula kwamangqamuzana ngenxa yenani eliningi loketshezi olungeziwe.