Ifekthri yamaseli iyisisetshenziswa sokwenza amaseli, esihlanganisa idivayisi yesiko lamaseli, ekwazi ukubona ubukhulu noma uhlobo lwamaseli amasiko eseli, futhi engabona ukusika okunembile kwamaseli, afanele izinkambu eziningi ezifana nezimboni zokwenza imithi.kunefekthri yeseli engungqimba engu-1, imboni yamaseli izendlalelo ezi-2, izendlalelo ezi-5 & izendlalelo eziyi-10 nezingqimba ezingama-40 ezitholakalayo.

1. Ngemva kokuba imboni yeseli ingene oketshezini, umlomo webhodlela wamukela umklamo womlomo obanzi, ongagcwalisa ngokushesha futhi uqoqe uketshezi, futhi akulula ukukhiqiza amabhamuza omoya.Ngesikhathi esifanayo, umklamo womlomo omkhulu uvumela kakhulu ukushintshaniswa kwegesi futhi ulungele isiko lamaseli aphezulu.

2. Imboni yamaseli ejwayelekile ifakwe amakepisi aphefumulayo angu-0.2m namakepisi angangeni moya, angasetshenziswa ezindaweni zamasiko ahlukene.Amakepisi angenawo umoya asetshenziswa ezindaweni ze-CO2, futhi amakepisi angangeni moya angasetshenziswa kuma-incubator avamile nasezindlini zokugcina izitshalo ezingenayo i-CO2.Ngaphezu kwalokho, isivalo se-liquid singaba ngokuzikhethela, esilungele ukuphakelwa kwe-aseptic liquid, futhi isivalo sebhodlela esilungele ukuphakelwa okuwuketshezi singenziwa ngokwezifiso ngokuya ngezidingo zenqubo yekhasimende.

3. Ifilimu ephefumulayo yesigqoko sebhodlela iklanyelwe i-hydrophobic, futhi ngeke ithinte ukuqina komoya kanye nomphumela wokungenisa umoya wefilimu ephefumulayo ngemva kokuxhumana noketshezi.

4. Inqubo yokunamathisela engenisiwe phakathi kwezimboni zamaseli ingamelana ne-1.5 PSI ukuze kuqinisekiswe ukuthi ukucindezela kongqimba ngalunye lomkhiqizo akuvuzi phakathi nayo yonke inqubo yokukhiqiza.

Isiko lamaseli liyasebenza nasekubambeleleni kwesiko lamaseli ngesikhathi sesiko lamaseli.I-Filamentous futhi ingena ngokushesha esigabeni sokukhula se-logarithmic.Ngokujwayelekile ngemva kwezinsuku ezimbalwa, indawo yesiko imbozwa ukuze yakhe i-monolayer eminyene yamaseli, njengamaseli e-Vero, amaseli we-HEK 293, amaseli e-CAR-T, i-MRC5, amaseli e-CEF, ama-macrophages e-alveolar , amaseli e-myeloma, amaseli e-DF-1, amaseli e-ST , amaseli e-PK15, amaseli e-Marc145, njll.