Siyabonaizimboni zamaselulaemikhakheni kusukela ekulungiseni umgomo kuya kuma-biopharmaceuticals.Iwumkhumbi we-multi-layer cell culture, onezinzuzo zokuthatha indawo encane kanye nezinga eliphezulu lokuvuna amaseli.Amaseli azwela kakhulu endaweni yawo, ngakho-ke kunezinto ezine okufanele uzinake lapho wenza lokhu:

1. Uma amaseli akhulisiwe, yonke imisebenzi kufanele yenziwe ngokuhambisana ngokuqinile nezidingo zokusebenza kwe-aseptic.

2. Sicela ushise kuqala i-imboni yamaselifuthi okuphakathi nendawo kuya ekushiseni kwesiko kusenesikhathi: Njengoba i-incubator inkulu, izoba yinde ukuthi ifinyelele izinga lokushisa elimisiwe lesiko, ngakho-ke ngaphambi kokuhlolwa, ukushisa kuqala imboni yeseli futhi okuphakathi nendawo kuya ekushiseni kwesiko kungasheshisa isivinini sokunamathela kweseli futhi ngcono kakhulu izinga lokuvuna amaseli.

3. ukusebenza kufanele kube mnene, gwema ukuzamazama okukhulu ukuze kukhiqizwe amabhamuza: amabhamuza angabangela ukugeleza kwemidiya kusuka kungqimba olungaphezulu kuya kwesendlalelo esingezansi, okuholela ekusakazweni kwemidiya okungalingani, ngisho nokuhlangana kweseli.

4. Gwema ukufutha utshwala noma isibulala-magciwane esembozweni esiphefumulayo.Utshwala noma isibulala-magciwane singamanzisa ulwelwesi lwesihlungi se-hydrophobic, kubangele kungabikho moya ophefumulayo, kuthinte ukushintshaniswa kwegesi noma kubangele ingcindezi engalingani ngesikhathi sokusebenza.

Lezi ngezinye zezinkinga okudingeka kugxilwe kuzo futhi kunakwe lapho kukhula amaseli kuimboni yamaseli.Ukwenziwa kwamaseli kuwumsebenzi onzima futhi onokucophelela, futhi ukunganaki okuncane kungase kubangele ukungcoliswa kwamaseli, ukugoqa, ukunganamatheli odongeni, njll. Kuphela ngokuqonda indlela yokusebenza efanele singaqinisekisa ukuqhubeka kahle kwesiko lamaseli.