Isiko lamaseli kuyindlela yokuthi amaseli aphile, akhule, akhiqize futhi agcine izakhiwo zawo eziyinhloko nemisebenzi ngokulingisa imvelo ku-vivo in vitro.Ibhodlela lamasiko eseliiwuhlobo lweseli olusebenzisekayo oluvame ukusetshenziswa kusiko lokunamathela kumaseli.Enqubweni yesiko lamaseli, sivame ukuthola ukunqwabelana kokungcola okuthile oketshezini.Kunezizathu eziningi zalesi simo, futhi izinga lokushisa libuye libe esinye sezizathu ezivamile.
Ukuba khona kwemvula kufulaski we-cell culture kungase kube umphumela wokungcoliswa kwamaseli.Uma ukungcola kukhishiwe, ukuthuthumela endaweni ye-cell culture ngokuvamile kuhunyushwa njengokuna kwezakhi zensimbi, amaprotheni, nezinye izingxenye eziphakathi nendawo.Iningi lemvula likhubaza ukwanda kwamaseli okuvamile ngoba lishintsha ukwakheka kwendawo ngokuchela imisoco nezinye izakhi ezidingekayo.Imvula ingase ibonwe nge-microscope futhi ingase iphazamise ukuhlolwa okudinga ukuhlaziywa kwesithombe.
 
Esikweni lamaseli, izinga lokushisa lingenye yezinto eziyinhloko ezibangela imvula.Lapho izinga lokushisa lishintsha ngokwedlulele, amaprotheni e-plasma anesisindo esikhulu samangqamuzana azodonswa esixazululweni.Ukungasebenzi kokushisa kanye nomjikelezo wokuncibilika kweqhwa kungakhuthaza ukuwohloka kwamaprotheni kanye nemvula.Ngenxa yokuthi uketshezi noma okushintshiwe kugcinwa endaweni ebandayo phakathi kokusetshenziswa, usawoti ungahlala, ikakhulukazi ku-10X noma ezinye izixazululo zesitoreji esigxilisiwe.
 
Yiqiniso, imvula ibonakala ebhodleleni le-cell culture.Uma kunqunywa ukuthi izinga lokushisa liyimbangela, kufanele kuqashelwe indawo yokugcina impahla kanye nendlela yokusebenza yendawo yesiko ukuze kugwenywe ukuqhwa okuphindaphindiwe nokuncibilika, okunganciphisa amathuba okuna.