Ierlenmeyer shake flaskiyisitsha esikhethekile samasiko esiko le-suspension cell culture, futhi singasetshenziswa ukulungisa, ukuxuba nokugcina imidiya ehlukahlukene.Lesi siko esisebenzisekayo sisetshenziswa lapho kutshalwa amaseli eJurkat.

I-Jurkat cell line isuselwa egazini le-peripheral lomfana oneminyaka engu-14 ubudala futhi iyiseli elilengayo.Imigqa yamaseli esuselwe ku-Jurkat engenazo izakhi zofuzo ezithile isivele itholakala emabhange e-cell culture.Imigqa ye-T lymphocyte engafi yabantu esetshenziselwa ikakhulukazi ukutadisha i-acute T cell leukemia, ukusayina kwamaseli e-T, kanye nama-chemokine receptors ahlukahlukene asengozini yokungena ngegciwane, ikakhulukazi isisho se-HIV.Inohlu olubanzi kakhulu lwezinhlelo zokusebenza ocwaningweni lwebhayoloji, njengokusetshenziswa kwamaseli e-Jurkat ukufunda i-M1-RNA ye-ribonuclease P, kanye nocwaningo lwe-M1-RNA ye-anti-MHC class II transcriptional activator (CIITA) ukuvimbela ukubonakaliswa kwama-molecule we-MHC class II endaweni yeseli.

Lapho uhlakulela amaseli e-Jurkat ku-erlenmeyer shake flasks, i-RPMI1640 medium, i-10% FBS iyadingeka;izinga lokushisa lilawulwa ku-37 ° C, 5% i-carbon dioxide, inani le-PH 7.2-7.4, isiko lokushisa elingaguquki le-aseptic.Sula futhi ubulale amagciwane ngotshwala obungama-75% ngaphambi kokudlulisela ebhentshini elihlanzekile kakhulu, khipha i-cell cryovial ethangini le-nitrogen eliwuketshezi, ngokushesha ulibeke kubhavu wamanzi ongu-37°C bese unyakazisa i-cryotube yeseli ngokushesha ukuze incibilike ngokushesha.Khona-ke, ngemva kwe-centrifugation, pipetting kanye nokuxuba, njll, yafakwa ku-incubator yeseli ukuze ilinywe.

Amaseli azwela kakhulu imvelo.Lapho uhlakulela amaseli e-Jurkat kuma-erlenmeyer cell shake flasks, inhlanzeko yomuntu siqu kufanele yenziwe kahle, ama-reagents oyinyumba kufanele asetshenziswe, futhi izimiso zokusebenza kwe-aseptic kufanele zilandelwe ukuze kugwenywe ukwethula amagciwane futhi kuthinte ukukhula kwamangqamuzana.