Ibhodlela lamasiko eseli  iwuhlobo lwesiko lamaseli olusebenzisekayo olusetshenziswa kakhulu ekuhloleni isiko lamaseli, futhi lusetshenziswa kakhulu kusiko lweseli lokubambelela.Esinye sezici ezibalulekile zokuhlola lolu hlobo lwezinto ezidliwayo yimpahla yokunamathela, ehlobene nokuthi amaseli angakwazi yini ukunamathela ebusweni bebhodlela kahle.

Amabhodlela amasiko amaseli  zenziwe nge-polystyrene, into ye-polymer esobala.Ngokusho kwezidingo ezihlukene zokukhula kwamaseli, afakwe ama-caps avaliwe ajwayelekile kanye nezihlungi ze-hydrophobic, ezingavimbela ngokuphumelelayo ukungcola ngenkathi zishintshanisa igesi.Ukusebenza kodonga lokunamathela kwebhodlela kuthinteka kakhulu yizici ezimbili:

1. Inqubo yokwelapha engaphezulu: Uma isetshenziselwa isiko lamaseli anamathelayo, ibhodlela le-cell culture lizophathwa okukhethekile ngaphambi kokusetshenziswa, futhi amaqembu e-hydrophilic azokwethulwa phezu kwebhodlela ukuqinisekisa ukuthi amaseli angakwazi ukunamathela phezulu ukuze akhule. .Uma ukusebenza kodonga lwebhodlela kukubi, kungase kube nezinkinga ekulawuleni imininingwane ekwelapheni okungaphezulu, futhi inqubo yokwelapha eqinile ingaqinisekisa umphumela wokunamathela odongeni womkhiqizo.
2. impahla yomkhiqizo: ngakolunye uhlangothi, ukusebenza kodonga nakho kuhlobene nezinto ezikhethwe umkhiqizo.Impahla yalokhu okusebenzisekayo kufanele ihlangabezane nezidingo ze-USPVI, okuwukuhlola okuqinile uma kuqhathaniswa kwempahla yepulasitiki emkhakheni wezokwelapha kanye nemikhiqizo yamapayipi emkhakheni we-biopharmaceutical.Ukuhlanzeka okusebenzisekayo okuhlangabezana nalesi simo kuphezulu uma kuqhathaniswa, okungathuthukisa ngokuyisisekelo ukusebenza kokunamathela komkhiqizo.

Lezi yizici ezimbili eziyinhloko ezithinta ukusebenza kokunamathela kwebhodlela le-cell culture.Ngaphezu kwalokho, uma ukusebenza kokunamathela kweseli kukubi, noma ngabe iseli ngokwalo lisesimeni esihle kufanele kucatshangelwe.Uma iseli lisesimweni esibi, umphumela walo wokunamathela nawo uzothinteka.

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