[Kopisha] I-Erlenmeyer Enobunxantathu Obunjwa Ngelaski enophawu noma ikepisi le-vent Isithombe Esifakiwe

[Kopisha] I-Erlenmeyer Enobunxantathu Obume Ngobunxantathu enophawu noma isivalo sokukhipha umoya

Amaflaski epulasitiki anomumo ongunxantathu we-LuoRon, adizayinelwe ukusetshenziswa kosikompilo lwamaseli.Yenziwe ngekhwalithi ephezulu ye-PETG ne-PC, lezi flasks ze-enlenmeyer zilungele kokubili isiko lamaseli anamathelayo kanye nokumiswa.Itholakala ngosayizi abane - 125ml, 250ml, 500ml kanye no-1000ml - ungathola kalula ivolumu esebenza kangcono ekuhloleni kwakho.Umumo oyingqayizivele ongunxantathu uvumela ukugeleza kwemidiya okuphelele nokuxubana okuphumelelayo, okukhuthaza ukukhula kwamaseli okunempilo.Lawa mabhodlela afika namakepisi angangenisi umoya ukuze avimbele ukungcola futhi aqinisekise indawo eyinyumba.Ngokubunjwa kwayo okusobala, inqubekelaphambili yesiko lamaseli ingaqashelwa kalula ngaphandle kokuphazamisa inqubo.Lawa mabhodlela aguquguqukayo ayisixazululo esiphezulu sesiko lamaseli angenamthungo, ukuhlanganisa ukusebenza, ukwethembeka kanye nokulula.Khuphula ucwaningo lwakho futhi uzizwele ileveli entsha yesiko lamaseli ngamaflaski ethu angama-triangular shaker epulasitiki.

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Imininingwane Yomkhiqizo

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I-Erlenmeyer Flask enekepisi yokukhipha umoya

Isici se-Erlenmeyer Shake Flask

I-erlenmeyer flask, eyaziwa nangokuthi i-triangular shake flask, ifaneleka kakhulu ukutshalwa kwemigqa yamaseli ezinambuzane ezinezidingo eziphezulu ze-oxygen.Uma kuqhathaniswa nezinto ezisetshenziswayo ezifana namafekthri amaseli kanye nama-cell spinner flasks, indawo yesiko lamaseli incane futhi iyithuluzi lezomnotho lamaseli..
Umzimba weflask wenziwe nge-polycarbonate (PC) noma i-PETG impahla.Idizayini eyingqayizivele yesimo esingunxantathu yenza kube lula ukuthi ama-pipette noma ama-cell scrapers afinyelele ekhoneni leflask, okwenza imisebenzi yesiko lamaseli ibe lula kakhulu.Isigqoko sebhodlela senziwe ngezinto eziqinile ze-HDPE, ezihlukaniswe zibe isivalo sokuvala kanye nesigqoko esiphefumulayo.I-cap yokuvala isetshenziselwa isiko elivaliwe legesi noketshezi.I-vent cap ifakwe ulwelwesi lwesihlungi se-hydrophobic phezulu kwekepisi yebhodlela.Ivimbela ukungena nokuphuma kwama-microorganisms, ivimbela ukungcola, futhi iqinisekise ukushintshaniswa kwegesi, ukuze amaseli noma amagciwane akhule kahle.

I-flask yesiko elingunxantathu lakhiwa umzimba webhodlela kanye nesigqoko sebhodlela .. Umklamo oyingqayizivele webhodlela lebhodlela lenza kube lula ukuthi ama-pipette noma ama-cell scrapers afinyelele ekhoneni lebhodlela, okwandisa ukunethezeka kokusebenza kwesiko lamaseli.kanye nokuzinza.Osayizi abajwayelekile bamaflask okunyakazisa angunxantathu ngu-125ml, 250ml, 500ml kanye no-1000ml.Ukuze ubuke umthamo we-medium futhi ubambe isimo sokukhula samaseli, isikali sizophrintwa emzimbeni webhodlela.Inqubo yeseli idinga ukwenziwa endaweni eyinyumba.Ngakho-ke, i-Erlenmeyer flask izophathwa ngokukhethekile inzalo ngaphambi kokuba isetshenziswe ukuze kuzuzwe umphumela wokungabi bikho kwe-DNase, i-RNase, kanye nezithako ezitholakala ezilwaneni, ezihlinzeka ngezimo ezinhle zokukhula kwamaseli.indawo ezungezile.

U-Erlenmeyer Shake onekepisi elikhipha umoya

Amaseli Akhula Kancane Kancane ku-Erlenmeyer Flask nesixazululo

Yini edala ukukhula kancane kwamaseli kumaflask e-cell shaker
Amaseli azwela kakhulu endaweni yokukhula.Lapho sihlakulela amangqamuzana, ngezinye izikhathi sibhekana nokukhula kwamaseli okuhamba kancane.Siyini isizathu?Kunezizathu eziningi zokukhula kancane kwamaseli ku-cell shake flask, ikakhulukazi ngenxa yalezi zizathu ezilandelayo:
1. Amaseli adinga ukulungiswa kabusha ngenxa yokushintsha kwesiko elihlukile noma i-serum.
2. Ama-reagents agcinwa ngendlela engafanele, futhi ezinye izingxenye ezidingekayo zokukhula kwamaseli njenge-glutamine noma izici zokukhula endaweni yesiko ziphelile noma azikho noma zicekelwe phansi.
3. Kukhona inani elincane lokungcola kwebhaktheriya noma ukukhunta esikweni ku-cell shaker.
4. Iqoqo lokuqala lamaseli afakwe i-inoculated liphansi kakhulu.
5. Amaseli asekhulile.
6. Ukungcola kwe-Mycoplasma
Isixazululo esiphakanyisiwe:
1. Qhathanisa ukwakheka kwendlela entsha kanye nendlela yokuqala, bese uqhathanisa i-serum entsha neserum endala ukuze usekele ukuhlolwa kokukhula kwamaseli.Vumela amaseli ukuthi ajwayelane kancane kancane nendawo entsha.
2. Shintshela endaweni yesiko elisanda kulungiswa, noma wengeze i-glutamine nezici zokukhula.
3. Fukamisa nge-antibiotic-free medium bese ubuyisela isiko uma ukungcola kutholakala.I-culture medium kufanele igcinwe ku-2-8 ° C ebumnyameni.Isilinganiso esiphelele esiqukethe i-serum sigcinwa ku-2-8°C futhi sisetshenziswe phakathi kwamaviki ama-2.
4. Khulisa ukugxila kokuqala kwamaseli afakwe i-inoculated.
5. Faka esikhundleni ngamaseli amasha anembewu.
6. Hlukanisa isiko futhi uthole i-mycoplasma.Hlanza i-stand kanye ne-incubator.Uma ukungcoliswa kwe-mycoplasma kutholakala, buyisela isiko elisha.

● Ipharamitha Yomkhiqizo

Isigaba	Inombolo ye-athikili	Ivolumu	Cap	Okubalulekile	Ukucaciswa kwephakheji	Ubukhulu bebhokisi
Erlenmeyer flask, PETG	LR030125	125ml	uphawu Cap	I-PETG,Ukuvala inzalo ngemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR030250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR030500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR030001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
Erlenmeyer flask, PETG	LR031125	125ml	I-Vent Cap	I-PETG,Ukuvala inzalo ngemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR031250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR031500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR031001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, i-PC	LR032125	125ml	uphawu Cap	I-PC, inzalo yokushiswa kwemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR032250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR032500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR032001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5
I-Erlenmeyer flask, i-PC	LR033125	125ml	I-Vent Cap	I-PC, inzalo yokushiswa kwemisebe	1pcs/pack24pack/case	31 X 21 X 22
	LR033250	250ml			1pcs/pack12pack/case	31 X 21 X 22
	LR033500	500ml			1pcs/pack12pack/case	43 X 32 X 22
	LR033001	1000ml			1pcs/pack12pack/case	55 X 33.7 X 24.5